LOCUS       sequence-260265-        1276 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            C-terminal V5-TurboID for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1276)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-hcRed-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..1276
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    135..159
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             162..845
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(847..871)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             873..914
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cgntgctgcg agtgtgtttt gtgagttttt taggcacctt ttgaatgtaa tcatttgggt
       61 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
      121 cttttttgtt agacgcaact ttgtacaaaa aagttggcac catggtgagc ggcctgctga
      181 aggagagtat gcgcatcaag atgtacatgg agggcaccgt gaacggccac tacttcaagt
      241 gcgagggcga gggcgacggc aaccccttcg ccggcaccca gagcatgaga atccacgtga
      301 ccgagggcgc ccccctgccc ttcgccttcg acatcctggc cccctgctgc gagtacggca
      361 gcaggacctt cgtgcaccac accgccgaga tccccgactt cttcaagcag agcttccccg
      421 agggcttcac ctgggagaga accaccacct acgaggacgg cggcatcctg accgcccacc
      481 aggacaccag cctggagggc aactgcctga tctacaaggt gaaggtgcac ggcaccaact
      541 tccccgccga cggccccgtg atgaagaaca agagcggcgg ctgggagccc agcaccgagg
      601 tggtgtaccc cgagaacggc gtgctgtgcg gccggaacgt gatggccctg aaggtgggcg
      661 accggcacct gatctgccac cactacacca gctaccggag caagaaggcc gtgcgcgccc
      721 tgaccatgcc cggcttccac ttcaccgaca tccggctcca gatgctgcgg aagaagaagg
      781 acgagtactt cgagctgtac gaggccagcg tggcccggta cagcgacctg cccgagaagg
      841 ccaacttgcc aactttcttg tacaaagtgg gcggcaagcc catccccaac cccctgctgg
      901 gcctggacag caccgctagc aaagacaata ctgtgcctct gaagctgatc gctctcctgg
      961 ctaatggcga gttccatagt ggcgaacagc tgggagaaac cctgggcatg tccaggggcc
     1021 gctatcaaca agcacattca gactctgcgc gactggggcg tgacgtgttc accgtgcccg
     1081 gaaaggctac tctctgcccg agccttatcc cgctgctgaa cgctaaacag attctgggac
     1141 agctggacgg cggagcgtgc agtctgctgt gncgactcca catcagtact gctgatcgat
     1201 cggccactga anggnagctg cattgccgna tatcagctta gtcaaggcac tgaagnnagc
     1261 caaaagggga ggnaaa
//
