LOCUS       sequence-260266-        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            C-terminal V5-TurboID for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-hcRed-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(341..382)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    384..408
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gncngtcgat tctgcatgca gcatccccac tcttcagctc gccgattcga tccagcaggt
       61 actgattggt ggagtcgacc acaggcagga ctgccacgct cccgccgtcc agctgtccca
      121 gaatctgttt agcgttcagc agcgggatag gctcgggcag agagtagccc tttccgggca
      181 cggtgaacac gtccacgccc cagtcgcgca gagtctgaat gtgcttgttg atagcggccc
      241 tggacatgcc cagggtttct cccagctgtt cgccactatg gaactcgcca ttagccagga
      301 gagcgatcag cttcagaggc acagtattgt ctttgctagc ggtgctgtcc aggcccagca
      361 gggggttggg gatgggcttg ccgcccactt tgtacaagaa agttggcaag ttggccttct
      421 cgggcaggtc gctgtaccgg gccacgctgg cctcgtacag ctcgaagtac tcgtccttct
      481 tcttccgcag catctggagc cggatgtcgg tgaagtggaa gccgggcatg gtcagggcgc
      541 gcacggcctt cttgctccgg tagctggtgt agtggtggca gatcaggtgc cggtcgccca
      601 ccttcagggc catcacgttc cggccgcaca gcacgccgtt ctcggggtac accacctcgg
      661 tgctgggctc ccagccgccg ctcttgttct tcatcacggg gccgtcggcg gggaagttgg
      721 tgccgtgcac cttcaccttg tagatcaggc agttgccctc caggctggtg tcctggtggg
      781 cggtcaggat gccgccgtcc tcgtaggtgg tggttctctc ccaggtgaag ccctcgggga
      841 agctctgctt gaagaagtcg gggatctcgg cggtgtggtg cacgaaagtc ctgctgccgt
      901 actcgcagca ggggccagga tgtcgaaagg cgaagggcaa gggggcgccc tcggtcacgt
      961 ggaatctcat gctctgggtg ccggcaaagg ggttgccgtc cccctcgccc tcgcacttga
     1021 aatatgacgt tcaccgtggc cttccatgta catctgatgc acatactctc ttcagcaggc
     1081 gctcacatgt gccaactttt ttgtacaagt tgcntctaac aaaagcaaaa ccgccggatt
     1141 tanggaacag ttactagtca acctgaaata cctaattg
//
