LOCUS       sequence-260268-        1231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            C-terminal V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1231)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-hcRed-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             163..847
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKGQ"
     protein_bind    complement(849..873)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             872..913
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(971..990)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gnagctggcg agtgtgtttt gtgagttttt taggcacctt ttgaaatgta atcatttggg
       61 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
      121 gcttttttgt tagacgcaac tttgtacaaa aaagttggca ccatggtgag cggcctgctg
      181 aaggagagta tgcgcatcaa gatgtacatg gagggcaccg tgaacggcca ctacttcaag
      241 tgcgagggcg agggcgacgg caaccccttc gccggcaccc agagcatgag aatccacgtg
      301 accgagggcg cccccctgcc cttcgccttc gacatcctgg ccccctgctg cgagtacggc
      361 agcaggacct tcgtgcacca caccgccgag atccccgact tcttcaagca gagcttcccc
      421 gagggcttca cctgggagag aaccaccacc tacgaggacg gcggcatcct gaccgcccac
      481 caggacacca gcctggaggg caactgcctg atctacaagg tgaaggtgca cggcaccaac
      541 ttccccgccg acggccccgt gatgaagaac aagagcggcg gctgggagcc cagcaccgag
      601 gtggtgtacc ccgagaacgg cgtgctgtgc ggccggaacg tgatggccct gaaggtgggc
      661 gaccggcacc tgatctgcca ccactacacc agctaccgga gcaagaaggc cgtgcgcgcc
      721 ctgaccatgc ccggcttcca cttcaccgac atccggctcc agatgctgcg gaagaagaag
      781 gacgagtact tcgagctgta cgaggccagc gtggcccggt acagcgacct gcccgagaaa
      841 ggccaacttg ccaactttct tgtacaaagt gggcaagcct atccctaacc ctctcctcgg
      901 tctcgattct acgtaataac actggatata ccaccgttga tatatcccaa tggcatcgta
      961 aagacatttt gaggcatttc agtcagttgc tcaatgtacc tataccagac cgttcagctg
     1021 gatattacgg ccttttaaga ccgtaagaaa atagcacagt ttatccggct ttatcacatc
     1081 tgcccgcctg taatcaactt atanacatag tgatatcagc acagtggcgc gctcgacatc
     1141 actctgatac aatngtnang attgactgnn tcctaacatg tgncntagcg ngtgaatcgc
     1201 ngctatgcct gtaataccnt gccaatgtgc t
//
