LOCUS       sequence-260269-        1228 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            C-terminal V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1228)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-hcRed-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1228
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..89
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     206..225
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(284..325)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    324..348
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcagtcatc tttccaaatt ttgtaatcca gaggttgatt gtcgagcggc cgccactgtg
       61 ctggatatca actatgtata ataaagttga ttacaggcgg gcaagaatgt gaataaaggc
      121 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
      181 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
      241 atgccattgg gatatatcaa cggtggtata tccagtgtta ttacgtagaa tcgagaccga
      301 ggagagggtt agggataggc ttgcccactt tgtacaagaa agttggcaag ttggccttct
      361 cgggcaggtc gctgtaccgg gccacgctgg cctcgtacag ctcgaagtac tcgtccttct
      421 tcttccgcag catctggagc cggatgtcgg tgaagtggaa gccgggcatg gtcagggcgc
      481 gcacggcctt cttgctccgg tagctggtgt agtggtggca gatcaggtgc cggtcgccca
      541 ccttcagggc catcacgttc cggccgcaca gcacgccgtt ctcggggtac accacctcgg
      601 tgctgggctc ccagccgccg ctcttgttct tcatcacggg gccgtcggcg gggaagttgg
      661 tgccgtgcac cttcaccttg tagatcaggc agttgccctc caggctggtg tcctggtggg
      721 cggtcaggat gccgccgtcc tcgtaggtgg tggttctctc ccaggtgaag ccctcgggga
      781 agctctgctt gaagaagtcg gggatctcgg cggtgtggtg cacgaaagtc ctgctgccgt
      841 actcgcagca ggggccagga tgtcgaaagc gaaagggcag gggggcgccc tcggtcacgt
      901 ggattctcat gctctgggtg ccggcgaaag ggttgccgtc gccctcgccc tcgcacttga
      961 agtagtgccg ttcacggtgc cctccatgta catcttgatg cgcatactct ctttcagcag
     1021 ccgctcacca tggtgctact tttgtacaag ttgcgtctaa caaaaagcca aacggcagat
     1081 ttagcgacat ttactagtct aacctgaaat acgattgaac cgaatgatac nttnnagtgc
     1141 taaaacttcc naaaacactc gcacccgagn agttcaaacc gantcgctnt agagatggta
     1201 ctacngacca gaacctggag ccctcact
//
