LOCUS       sequence-260277-        1249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of GFP (as control) tagged with C-terminal
            V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1249)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-GFP-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    133..157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(178..180,181..183,184..894)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(223..244)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(484..503)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     831..852
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(914..938)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             937..978
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(1037..1056)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gggctggcgg tgtgttttgt gagtttttta ggcacctttt gaatgtaatc atttgggtca
       61 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
      121 tttttgttag acgcaacttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg
      181 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      241 gacgtaaacg gccacaagtt cagcgtgtct ggcgagggcg agggcgatgc cacctacggc
      301 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      361 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
      421 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
      481 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
      541 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
      601 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
      661 atcaaggcga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
      721 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      781 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
      841 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccaag
      901 ggtgggcgcg ccgacccagc tttcttgtac aaagtgggca agcctatccc taaccctctc
      961 ctcggtctcg attctacgta ataacactgg atataccacc gttgatatat cccaatggca
     1021 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     1081 tcagctggat atttacggcc tttttcaaag acggtaaaga aaatagcaca aggttttatt
     1141 ccggcnttta tccatctgcc gcctgtaatc aacttattta cataggtgaa ttccagncnn
     1201 gcgngnncgc aatcactctg atacaaattg ggaaagaatt gcactggna
//
