LOCUS       sequence-260278-        1230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of GFP (as control) tagged with C-terminal
            V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1230)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-GFP-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..91
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     208..227
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(286..327)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    326..350
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(412..433)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     761..780
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tnaccagctc atctttccaa ttttgtaatc cagaggttga ttgtcgagcg gccgccactg
       61 tgctggatat caactatgta taataaagtt gattacaggc gggcaagaat gtgaataaag
      121 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      181 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      241 cgatgccatt gggatatatc aacggtggta tatccagtgt tattacgtag aatcgagacc
      301 gaggagaggg ttagggatag gcttgcccac tttgtacaag aaagctgggt cggcgcgccc
      361 acccttggac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      421 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
      481 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      541 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcgcctt
      601 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
      661 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
      721 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
      781 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
      841 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtatg tcaggtggtc
      901 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt caggtcagct
      961 tgccgtagtg gcatcgccct cgccctcgcc agacacgctg aacttgtgcc gtttacgtcg
     1021 cgtcagctcg accaggatgg gcaccacccc ggtgacagct cctcgccctg ctcaccatgt
     1081 gaggggcgcc gcggagcctg ctttttttgt acaagtgcgt ctaacaaaaa agtccaaacg
     1141 cggaatacgg acattactat gtctacctga aataccattg accatgatac ttcaaggtct
     1201 aaactncaan actgcaacct gagcgccata
//
