LOCUS       sequence-26028-e         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Inoue T, Heo WD, Grimley JS, Wandless TJ, Meyer T
  TITLE     An inducible translocation strategy to rapidly activate and inhibit 
            small GTPase signaling pathways.
  JOURNAL   Nat Methods. 2005 Jun . 2(6):415-8.
  PUBMED    15908919
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2005 Jun . 2(6):415-8."
COMMENT     Sequence Label: YFP-tagged FRB (YR)
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    24..89
                     /label=MCS
                     /note="multiple cloning site"
     CDS             102..380
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     polyA_signal    498..619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(535..554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     589..608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcggcntgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattctg
       61 cagtcgacgg taccgcgggc ccgggatcca ccggatctag aatcctctgg catgagatgt
      121 ggcatgaagg cctggaagag gcatctcgtt tgtactttgg ggaaaggaac gtgaaaggca
      181 tgtttgaggt gctggagccc ttgcatgcta tgatggaacg gggcccccag actctgaagg
      241 aaacatcctt taatcaggcc tatggtcgag atttaatgga ggcccaagag tggtgcagga
      301 agtacatgaa atcagggaat gtcaaggacc tcctccaagc ctgggacctc tattatcatg
      361 tgttccgacg aatctcaaag actagataac tgatcataat cagccatacc acatttgtag
      421 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      481 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      541 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      601 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
      661 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
      721 tataaatcaa aanaatagan cgaganaggg ttgantgttg ttccnnttgg a
//
