LOCUS       sequence-260284-        1163 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of CDK12 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1163)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-CDK12-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..1163
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(342..383)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    385..409
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggcgtcgaa ttctgcatgc agcatcccca ctcttcagct cgccgattcg atccagcagg
       61 tactgattgg tggagtcgac cacaggcagg actgccacgc tcccgccgtc cagctgtccc
      121 agaatctgtt tagcgttcag cagcgggata ggctcgggca gagagtagcc ctttccgggc
      181 acggtgaaca cgtccacgcc ccagtcgcgc agagtctgaa tgtgcttgtt gatagcggcc
      241 ctggacatgc ccagggtttc tcccagctgt tcgccactat ggaactcgcc attagccagg
      301 agagcgatca gcttcagagg cacagtattg tctttgctag cggtgctgtc caggcccagc
      361 agggggttgg ggatgggctt gccgcccact ttgtacaaga aagctgggtt gccgctcgag
      421 cctgagtaag gaactcctct ccctcttccc cctcttggtg ggactcttgt aggcccccga
      481 tagagttttc cataagcaga agactgagtt gggccccccc agtgaaggcc tgctcctgag
      541 ctgctggccc cagtggttcc tggccccagc tccccatagt tttgcaattt ggtctctgca
      601 tgtaccacag aattgctgct tccctcagcc ttcaggaatg gttgcccgac caccgggtgt
      661 aacgctaagg ggaccctggc aaaacggagg tcctggtccc ctggaaactg cactgaccct
      721 gtgccctggt aactgctgga ctccccaagg tggctcagag agcctgagaa ggtcctgttc
      781 ttcaccaggg tctggaccaa ggattctgtc aaggctggac cagagtttcg ttcatcagtg
      841 tcaatggcac tgaaccctgt ttcagcccat cagtgtttcc ataagtcctg tttggacggg
      901 gtcgggtgga gtactccatt ggtctcaagg cctggtgctc atgtggcagt ggccaggagg
      961 ctctgcttca agctgggata aaagttgcag caaggcggct gtcacggctg ggtcaactcc
     1021 ttggggggcg ctggaaagat cgtcttcaac agagggggtg gagtgcggcg tgagtcaggg
     1081 ggctcagggg ctccttctct gctgctcttc tgtgcattgt gggagttctt cgggccctgg
     1141 tgcccactgt ttctttgtca ctg
//
