LOCUS       sequence-260287-        1252 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of CDK12 (human) tagged with C-terminal V5
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1252)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-CDK12-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1252
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..91
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     208..227
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(286..327)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    326..350
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttcaagctc atctttccaa ttttgtaatc cagaggttga ttgtcgagcg gccgccactg
       61 tgctggatat caactatgta taataaagtt gattacaggc gggcaagaat gtgaataaag
      121 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
      181 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
      241 cgatgccatt gggatatatc aacggtggta tatccagtgt tattacgtag aatcgagacc
      301 gaggagaggg ttagggatag gcttgcccac tttgtacaag aaagctgggt tgccgctcga
      361 gcctgagtaa ggaactcctc tccctcttcc ccctcttggt gggactcttg taggcccccg
      421 atagagtttt ccataagcag aagactgagt tgggcccccc cagtgaaggc ctgctcctga
      481 gctgctggcc ccagtggttc ctggccccag ctccccatag ttttgcaatt tggtctctgc
      541 atgtaccaca gaattgctgc ttccctcagc cttcaggaat ggttgcccga ccaccgggtg
      601 taacgctaag gggaccctgg caaaacggag gtcctggtcc cctggaaact gcactgaccc
      661 tgtgccctgg taactgctgg actccccaag gtggctcaga gagcctgaga aggtcctgtt
      721 cttcaccagg gtctggacca aggattctgt caaggctgga ccagagtttc gttcatcagt
      781 gtcaatggca ctgaaccctg tttcacgccc atcagtgttt ccataagtcc tgtttggacg
      841 gggtcgggtg gagtactcca ttggtctcaa ggcctggtgc tcatgtggca gtggccagga
      901 ggctctgctt cagctgggaa aaagttgcag caaggcggct gtcacggctg ggttcaactc
      961 ctgggggcgc tggaaagatc gccttcaacc agaggggtgg agtggcgcgg tggagtccgg
     1021 ggggctcagg gggcctcttc tctgctgctc tctgtggcat tgtgggagtt cttcggggcc
     1081 ctgtgccact gttcttgtca ctgttgtttc tcagactgct gctgcttctt ggatttcatc
     1141 actgactcag agacctgcat aattctgcat gtcagctgnt gcctgaagct caaggncatc
     1201 tgntcgctga ggcaggatac atgagnnang gngcctctct ctcaaggact cc
//
