LOCUS       sequence-260289-        1225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1225)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PKMYT1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gntggctggc gagtgtgttt tgtgagtttt ttaggcacct tttgaaatgt aatcatttgg
       61 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt
      121 ggcttttttg ttagacgcaa ctttgtacaa aaaagttggc accatgctag aacggcctcc
      181 tgcactggcc atgcccatgc ccacggaggg caccccgcca cctctgagtg gcacccccat
      241 cccagtccca gcctacttcc gccacgcaga acctggattc tccctcaaga ggcccagggg
      301 gctcagccgg agcctcccac ctccgccccc tgccaagggc agcattccca tcagccgcct
      361 cttccctcct cggaccccag gctggcacca gctgcagccc cggcgggtgt cattccgggg
      421 cgaggcctca gagactctgc agagccctgg gtatgaccca agccggccag agtccttctt
      481 ccagcagagc ttccagaggc tcagccgcct gggccatggc tcctacggag aggtcttcaa
      541 ggtgcgctcc aaggaggacg gccggctcta tgcggtaaag cgttccatgt caccattccg
      601 gggccccaag gaccgggccc gcaagttggc cgaggtgggc agccacgaga aggtggggca
      661 gcacccatgc tgcgtgcggc tggagcaggc ctgggaggag ggcggcatcc tgtacctgca
      721 gacggagctg tgcgggccca gcctgcagca acactgtgag gcctggggtg ccagcctgcc
      781 tgaggcccag gtctggggct acctgcggga cacgctgctt gccctggccc atctgcacag
      841 ccagggcctg gtgcaccttg atgtcaagcc tgccaacatc ttcctggggc cccggggccg
      901 ctgcaagctg ggtgacttcg gactgctggt ggagctgggt acagcagagc tggtgaggtc
      961 caggaggaga ccccgctaca tggccccgag ctgctgcagg gctctatggg acagcagcgg
     1021 atgtgttcag tctgggctca catctggaag tggcatgcac atggagctgc ccacggtggg
     1081 gagggctgca gcagctgcgc aggctactgc ccctgagtcc tgcgtctgtt cttcgagctg
     1141 nttctgtctg tcatgatgct gaccgaacca gctgccgncc aagccancng ctgncatgct
     1201 gttttgagca ccgcgcntgt gnnnt
//
