LOCUS       sequence-260292-        1222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1222)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PKMYT1-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..1222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gnnntggctg cggtgtgttt tgtgagtttt ttaggcacct tttgaatgta atcatttggg
       61 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
      121 gcttttttgt tagacgcaac tttgtacaaa aaagttggca ccatgctaga acggcctcct
      181 gcactggcca tgcccatgcc cacggagggc accccgccac ctctgagtgg cacccccatc
      241 ccagtcccag cctacttccg ccacgcagaa cctggattct ccctcaagag gcccaggggg
      301 ctcagccgga gcctcccacc tccgccccct gccaagggca gcattcccat cagccgcctc
      361 ttccctcctc ggaccccagg ctggcaccag ctgcagcccc ggcgggtgtc attccggggc
      421 gaggcctcag agactctgca gagccctggg tatgacccaa gccggccaga gtccttcttc
      481 cagcagagct tccagaggct cagccgcctg ggccatggct cctacggaga ggtcttcaag
      541 gtgcgctcca aggaggacgg ccggctctat gcggtaaagc gttccatgtc accattccgg
      601 ggccccaagg accgggcccg caagttggcc gaggtgggca gccacgagaa ggtggggcag
      661 cacccatgct gcgtgcggct ggagcaggcc tgggaggagg gcggcatcct gtacctgcag
      721 acggagctgt gcgggcccag cctgcagcaa cactgtgagg cctggggtgc cagcctgcct
      781 gaggcccacg tctggggcta cctgcgggac acgctgcttg ccctggccca tctgcacagc
      841 cagggcctgg tgcaccttga tgtcaagcct gccaacatct tcctggggcc ccggggccgc
      901 tgcaagctgg gtgacttcgg actgctggtg gagctgggta cagcaggagc tggtgaggtc
      961 caggagggag acccccgcta catgggcccc gagctgctgc aggctcctat gggacagcag
     1021 cggatgttgt tcagtctggg cctcaccaat cctggaagtg catgcacatg gagctgccca
     1081 cgggtggggg agggctggca gcactgccca ggctaactgc cctggagtca ctgcgnctgt
     1141 cctcactgcg tcgtcctgnc atgatgctgg aaccgaaccc aagctgccgc nangtcagag
     1201 acctggctgg nncatggccn gt
//
