LOCUS       sequence-260293-        1205 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1205)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PKMYT1-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..1205
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(341..382)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    384..408
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggnngtcgat tctgcatgca gcatccccac tcttcagctc gccgattcga tccagcaggt
       61 actgattggt ggagtcgacc acaggcagga ctgccacgct cccgccgtcc agctgtccca
      121 gaatctgttt agcgttcagc agcgggatag gctcgggcag agagtagccc tttccgggca
      181 cggtgaacac gtccacgccc cagtcgcgca gagtctgaat gtgcttgttg atagcggccc
      241 tggacatgcc cagggtttct cccagctgtt cgccactatg gaactcgcca ttagccagga
      301 gagcgatcag cttcagaggc acagtattgt ctttgctagc ggtgctgtcc aggcccagca
      361 gggggttggg gatgggcttg ccgcccactt tgtacaagaa agttggggtt gggtctaggg
      421 tgtcctcaaa caggctgagg aggttccgag gctcaaagga ggggaaggag ccccgaggag
      481 gctctgagtt gatgtcactt aggtccaggg catccctggg tgtgcacctg ctccgggggg
      541 tggaggtgct ccccacagtc cgggccagga cagcctcagg ggagagtgaa ggccctaggc
      601 tgtcgtcatc ccagttgctg gagaggctgc tgtccaggag caaactgcag ggtggtgagc
      661 caggcggggt ggctggcggg cccaggggct gtagccagct ggcagggtga gccagcccat
      721 gccagagcca gcagagcagg gcaagcaggg cctgccacag ggcccaccct cggctcaggc
      781 ctccgctgcc atgcaccaca gcacacccca ggcccgcggc tgcctcaaca caggcagtgc
      841 cagcaggcct cggccgtggc ccgcagcttg gggtctggct ccagcatcat gacaaagaca
      901 gaacgcagct cggaagacag accggcagtg aactcagggg gcaggtagcc ctggcgcagc
      961 tgctgccagc cctccccacc ggtgggggca gctccatgtt gcatgcactt ccaggatgtg
     1021 agcccagact gaacaacatc cgctgctgtc ccataggagc cctgcagcag ctcgggggcc
     1081 atgtaacggg ggtcttcctt cttggacctc acagctctgc tgtacccagc tcaacagcag
     1141 tcgagttcac cagctgcaac ggaccnggga cccaaggaag atgttggcaa ggctttgaca
     1201 tcnaa
//
