LOCUS       sequence-260295-        1196 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with C-terminal 
            V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1196)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PKMYT1-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1196
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    134..158
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gnngatgcga gtgtgttttg tgagtttttt aggcaccttt tgaatgtaat catttgggtc
       61 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
      121 ttttttgtta gacgcaactt tgtacaaaaa agttggcacc atgctagaac ggcctcctgc
      181 actggccatg cccatgccca cggagggcac cccgccacct ctgagtggca cccccatccc
      241 agtcccagcc tacttccgcc acgcagaacc tggattctcc ctcaagaggc ccagggggct
      301 cagccggagc ctcccacctc cgccccctgc caagggcagc attcccatca gccgcctctt
      361 ccctcctcgg accccaggct ggcaccagct gcagccccgg cgggtgtcat tccggggcga
      421 ggcctcagag actctgcaga gccctgggta tgacccaagc cggccagagt ccttcttcca
      481 gcagagcttc cagaggctca gccgcctggg ccatggctcc tacggagagg tcttcaaggt
      541 gcgctccaag gaggacggcc ggctctatgc ggtaaagcgt tccatgtcac cattccgggg
      601 ccccaaggac cgggcccgca agttggccga ggtgggcagc cacgagaagg tggggcagca
      661 cccatgctgc gtgcggctgg agcaggcctg ggaggagggc ggcatcctgt acctgcagac
      721 ggagctgtgc gggcccagcc tgcagcaaca ctgtgaggcc tggggtgcca gcctgcctga
      781 ggcccatgtc tggggctacc tgcgggacac gctgcttgcc ctggcccatc tgcacagcca
      841 gggcctggtg caccttgatg tcaagcctgc caacatcttc ctggggcccc ggggccgctg
      901 caagctgggt gacttcggac tgctggtgga gctgggtaca gcagagctgg tgaggtccag
      961 gagggagacc cccgctacat ggcccccgag ctgctgcagg ctcctatggg acagcagcga
     1021 tgtgtcagtc tgggctcaca tcctggaagt gcatgcaaca tgagctgccc acgggtgggg
     1081 gaaggctgca gcagctgcta gggctactgc ccctggagtc anngcgnctg tctcggctgc
     1141 nnttctgtct ggcatgatgc tggancnacc cagcctgcnn ncaagggccg agncct
//
