LOCUS       sequence-260296-        1161 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of PKMYT1 (human) tagged with C-terminal 
            V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1161)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-PKMYT1-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1161
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..87
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     204..223
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(282..323)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    322..346
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cngctcatct ttccaatttt gtaatccaga ggttgattgt cgagcggccg ccactgtgct
       61 ggatatcaac tatgtataat aaagttgatt acaggcgggc aagaatgtga ataaaggccg
      121 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
      181 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
      241 gccattggga tatatcaacg gtggtatatc cagtgttatt acgtagaatc gagaccgagg
      301 agagggttag ggataggctt gcccactttg tacaagaaag ttggggttgg gtctagggtg
      361 tcctcaaaca ggctgaggag gttccgaggc tcaaaggagg ggaaggagcc ccgaggaggc
      421 tctgagttga tgtcacttag gtccagggca tccctgggtg tgcacctgct ccggggggtg
      481 gaggtgctcc ccacagtccg ggccaggaca gcctcagggg agagtgaagg ccctaggctg
      541 tcgtcatccc agttgctgga gaggctgctg tccaggagca aactgcaggg tggtgagcca
      601 ggcggggtgg ctggcgggcc caggggctgt agccagctgg cagggtgagc cagcccatgc
      661 cagagccagc agagcagggc aagcagggcc tgccacaggg cccaccctcg gctcagggcc
      721 tccgctgcca tgcaccacag cacaccccag gcccgcggct gcctcaacac aggcagtgcc
      781 agcagggcct cggccgtggc ccgcagcttg gggtctggct ccagcatcat gacaaggaca
      841 gaacgcagct cggaagacag accggcagtg aactcagggg gcaggtagcc ctggcgcagc
      901 tgctgccagc tctccccacc gtggggcagc tccatgttgc atgccacttc caggatggtg
      961 aggcccagac tgaacacatc cgctgctgtc ccataggagc cctgcagcag ctcgggggcc
     1021 atggtagcgg ggggtctccc tctggacctc acagctctgc tgtacccagc tccacagcag
     1081 tcgagtcacc cagcttgcan gggccngnnt ccaggaagat gtttggcagg cttganncaa
     1141 gggtgcacca aggccctggc c
//
