LOCUS       sequence-260298-        1218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1218)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TLK2-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    140..164
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(742..753)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gngnctggct ggcgagtgtg ttttgtgagt tttttaggca ccttttgaaa tgtaatcatt
       61 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt
      121 tttggctttt ttgttagacg caactttgta caaaaaagtt ggcatggaag aattgcatag
      181 cctggaccca cgacggcagg aattattgga ggccaggttt actggagtag gtgttagtaa
      241 gggaccactt aatagtgagt cttccaacca gagcttgtgc agcgtcggat ccttgagtga
      301 taaagaagta gagactcccg agaaaaagca gaatgaccag cgaaatcgga aaagaaaagc
      361 tgaaccatat gaaactagcc aagggaaagg cactcctagg ggacataaaa ttagtgatta
      421 ctttgagttt gctgggggaa gcgcgccagg aaccagccct ggcagaagtg ttccaccagt
      481 tgcacgatcc tcaccgcaac attccttatc caatccctta ccgcgacgag tagaacagcc
      541 cctctatggt ttagatggca gtgctgcaaa ggaggcaacg gaggagcagt ctgctctgcc
      601 aaccctcatg tcagtgatgc tagcaaaacc tcggcttgac acagagcagc tggcgcaaag
      661 gggagctggc ctctgcttca cttttgtttc agctcagcaa aacagtccct catctacggg
      721 atctggcaac acagagcatt cctgcagctc ccaaaaacag atctccatcc agcacagaca
      781 gacccagtcc gacctcacaa tagaaaaaat atctgcacta gaaaacagta agaattctga
      841 cttagagaag aagagggaag aatagatgat ttattaagag ccaactgtga tttgagacgg
      901 cagattgatg aacagcaaaa gatgctagag aaatacaagg aacgattaaa tagatgtgtg
      961 acaatgagca agaaactcct tatagaaaag tcaaaacaga gaagatggcg tgtagagata
     1021 gagcatgcag accgcttgag actgggcact ttactactgt ccgacacgga gcctcattta
     1081 ctgaacagtg gacagatggt tatgcttttt cagatcttat cagcacagna agntaatcca
     1141 gagggaagag atgaagacac gaatgttagc aggcgaactc tgcatggnca gcccttcttg
     1201 caccatgtga cgaaaacg
//
