LOCUS       sequence-260299-        1229 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1229)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TLK2-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1229
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(159..200)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    202..226
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cncncggcct taattctgca tgcagcatcc ccactcttca gctcgccgat tcgatccagc
       61 aggtactgat tggtggagtc gaccacaggc aggactgcca cgctcccgcc gtccagctgt
      121 cccagaatct gtttagcgtt cagcagcggg atgctagcgg tgctgtccag gcccagcagg
      181 gggttgggga tgggcttgcc gcccactttg tacaagaaag ttgggcaatt agaagaactg
      241 ttattggacg ccccagaggt tgatgcaata gcagctccag cagggctact tgtagagact
      301 gactttcgga tgtgaggcaa caagtaggga tcacaggcca gctgctggac atcaatgcgg
      361 tcctcctttc ggtaggccaa gcatcgtcga ataaacgcct ttgcttcagg tgttactact
      421 ggctttggcg ggaactgcac ttcagtagct ttaagaatcg tattctcttg taggatgtct
      481 tgctgagact ggttatggcc aaaaggcttc cttccataaa gacactgata gaagatcaca
      541 cccaccgacc acacatcaac tttatttgag atctttggtg gttctttccc aaccacaaaa
      601 cactctggtg gtaaatacca ataagtacca gcaccttgtg atgttagctc catgccatcc
      661 actgaattgt agctatcatc atccatgatc ttcgaaagac caaaatctgt aatttttatc
      721 tctccacacg ctgtaccatt tactaaaaga atattacctg gtttgaggtc atagtgtatg
      781 atgggaggtt ttatttcatt taagtacttt aaagcattca caatctgcat gataatggac
      841 cgggcctctt tctccgacat taatttgtgc tgtttcacgt acaagtccag atcatttccc
      901 tcacagtatt ctaatactgt acaaaacgaa gtcataatcc agtgaaaagt aatcattaca
      961 gcttatctat tctgggatga tccatctcct ttatgaatcc gggatttcct acctgcatgc
     1021 ctggggtaaa tctcctattt cctcacctct ccagttttat attgactggt gaaattttac
     1081 acactacgta tctttgcttt gttagatcaa tgcctggaaa ctttcactga accttcctct
     1141 cnaaagatta acgccaaata ctccgtgcat gtgacggtgg agatctttaa ngagaattta
     1201 ccctcactat agtgcatccc ctatttaga
//
