LOCUS       sequence-260301-        1173 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1173)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TLK2-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..1173
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(739..750)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gnnctgctgc gagtgtgttt tgtgagtttt ttaggcacct tttgaaatgt aatcatttgg
       61 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt
      121 ggcttttttg ttagacgcaa ctttgtacaa aaaagttggc atggaagaat tgcatagcct
      181 ggacccacga cggcaggaat tattggaggc caggtttact ggagtaggtg ttagtaaggg
      241 accacttaat agtgagtctt ccaaccagag cttgtgcagc gtcggatcct tgagtgataa
      301 agaagtagag actcccgaga aaaagcagaa tgaccagcga aatcggaaaa gaaaagctga
      361 accatatgaa actagccaag ggaaaggcac tcctagggga cataaaatta gtgattactt
      421 tgagtttgct gggggaagcg cgccaggaac cagccctggc agaagtgttc caccagttgc
      481 acgatcctca ccgcaacatt ccttatccaa tcccttaccg cgacgagtag aacagcccct
      541 ctatggttta gatggcagtg ctgcaaagga ggcaacggag gagcagtctg ctctgccaac
      601 cctcatgtca gtgatgctag caaaacctcg gcttgacaca gagcagctgg cgcaaagggg
      661 agctggcctc tgcttcactt ttgtttcagc tcagcaaaac agtccctcat ctacgggatc
      721 tggcaacaca gagcattcct gcagctccca aaaacagatc tccatccagc acagacagac
      781 ccagtccgac ctcacaatag aaaaaatatc tgcactagaa aacagtaaga attctgactt
      841 agagaagatg agggaagaat agatgattta ttaagagcca actgtgattt gagacggcag
      901 attgatgaac agcaaagatg ctagagaaat acaaggacga ttaaatagat gtgtgacatg
      961 agcagaaact ccttatagaa aagtcaaaac aagagagatg gcgtgtagag atagagcatg
     1021 cagaccgctt gagactgggc cactttacta ctgtcgaccg agcctcatta ctgaacagtg
     1081 acgatgttat gctttcgatc tatcagcaac gagnnnntcc agaggaagaa atagaagacc
     1141 acgaaatgtt agcaagcgaa cctctcctgc cca
//
