LOCUS       sequence-260302-        1206 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1206)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TLK2-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..1206
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(345..386)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    388..412
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggccntct aatttctgca tgcagcatcc ccactcttca gctcgccgat tcgatccagc
       61 aggtactgat tggtggagtc gaccacaggc aggactgcca cgctcccgcc gtccagctgt
      121 cccagaatct gtttagcgtt cagcagcggg ataggctcgg gcagagagta gccctttccg
      181 ggcacggtga acacgtccac gccccagtcg cgcagagtct gaatgtgctt gttgatagcg
      241 gccctggaca tgcccagggt ttctcccagc tgttcgccac tatggaactc gccattagcc
      301 aggagagcga tcagcttcag aggcacagta ttgtctttgc tagcggtgct gtccaggccc
      361 agcagggggt tggggatggg cttgccgccc actttgtaca agaaagttgg gcaattagaa
      421 gaactgttat tggacgcccc agaggttgat gcaatagcag ctccagcagg gctacttgta
      481 gagactgact ttcggatgtg aggcaacaag tagggatcac aggccagctg ctggacatca
      541 atgcggtcct cctttcggta ggccaagcat cgtcgaataa acgcctttgc ttcaggtgtt
      601 actactggct ttggcgggaa ctgcacttca gtagctttaa gaatcgtatt ctcttgtagg
      661 atgtcttgct gagactggtt atggccaaaa ggcttccttc cataaagaca ctgatagaag
      721 atcacaccca ccgaccacac atcaacttta tttgagatct ttggtggttc tttcccaacc
      781 acaaaacact ctggtggtaa ataccaataa gtaccagcac cttgtgatgt tagctccatg
      841 ccatccactg aattgtagct atcatcatcc atgatcttcg aaagaccaaa atctgtaatt
      901 tttatctctc cacacgctgt accatttact aaaagaatat tacctgggtt tgaggtcata
      961 gtgtatgatg ggaggtttta tttcatttaa gtactttaaa gcattcacaa tctgcatgat
     1021 aatggaccgg gcctctttct ccgacattaa tttgtgctgt ttcaggtaga agtccagatc
     1081 atttccctca cagtattcta atactgtaca aacgagtcag tatcantgaa gtatcataca
     1141 gctactattc tggatgatca gcttntttta ttgatccggt attcccctaa catgcatgcc
     1201 tttggt
//
