LOCUS       sequence-260304-        1230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with C-terminal V5 
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1230)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TLK2-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    137..161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(739..750)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gncctggctg cggtgtgttt tgtgagtttt ttaggcacct tttgaaatgt aatcatttgg
       61 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt
      121 ggcttttttg ttagacgcaa ctttgtacaa aaaagttggc atggaagaat tgcatagcct
      181 ggacccacga cggcaggaat tattggaggc caggtttact ggagtaggtg ttagtaaggg
      241 accacttaat agtgagtctt ccaaccagag cttgtgcagc gtcggatcct tgagtgataa
      301 agaagtagag actcccgaga aaaagcagaa tgaccagcga aatcggaaaa gaaaagctga
      361 accatatgaa actagccaag ggaaaggcac tcctagggga cataaaatta gtgattactt
      421 tgagtttgct gggggaagcg cgccaggaac cagccctggc agaagtgttc caccagttgc
      481 acgatcctca ccgcaacatt ccttatccaa tcccttaccg cgacgagtag aacagcccct
      541 ctatggttta gatggcagtg ctgcaaagga ggcaacggag gagcagtctg ctctgccaac
      601 cctcatgtca gtgatgctag caaaacctcg gcttgacaca gagcagctgg cgcaaagggg
      661 agctggcctc tgcttcactt ttgtttcagc tcagcaaaac agtccctcat ctacgggatc
      721 tggcaacaca gagcattcct gcagctccca aaaacagatc tccatccagc acagacagac
      781 ccagtccgac ctcacaatag aaaaaatatc tgcactagaa aacagtaaga attctgactt
      841 agagaagaag agggaagaat agatgattta ttaagagcca actgtgattt gagacggcag
      901 attgatgaac agcaaaagat gctagagaaa tacaaggaac gattaaatag atgtgtgaca
      961 atgagcaaga aactccttat agaaaagtca aaacaggaga gatggcgtgt agagatagag
     1021 catgcagacc gcttgagact gggccacttt actactgtcc gacacggagc ctcatttact
     1081 gaacaggtga cagatggtta tgctttcaga atctttatca gcaacaggaa aggataaatt
     1141 caagaggana gaatgaaaga cacggaaatg gtagccagcg ganctctgca tggntcagnc
     1201 cttctgcaca atgannnaaa aacagccgga
//
