LOCUS       sequence-260305-        1208 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of TLK2 (human) tagged with C-terminal V5 
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1208)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-TLK2-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1208
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..90
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     207..226
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(285..326)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    325..349
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 antccagtca tctttccaat tttgtaatcc agaggttgat tgtcgagcgg ccgccactgt
       61 gctggatatc aactatgtat aataaagttg attacaggcg ggcaagaatg tgaataaagg
      121 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
      181 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
      241 gatgccattg ggatatatca acggtggtat atccagtgtt attacgtaga atcgagaccg
      301 aggagagggt tagggatagg cttgcccact ttgtacaaga aagttgggca attagaagaa
      361 ctgttattgg acgccccaga ggttgatgca atagcagctc cagcagggct acttgtagag
      421 actgactttc ggatgtgagg caacaagtag ggatcacagg ccagctgctg gacatcaatg
      481 cggtcctcct ttcggtaggc caagcatcgt cgaataaacg cctttgcttc aggtgttact
      541 actggctttg gcgggaactg cacttcagta gctttaagaa tcgtattctc ttgtaggatg
      601 tcttgctgag actggttatg gccaaaaggc ttccttccat aaagacactg atagaagatc
      661 acacccaccg accacacatc aactttattt gagatctttg gtggttcttt cccaaccaca
      721 aaacactctg gtggtaaata ccaataagta ccagcacctt gtgatgttag ctccatgcca
      781 tccactgaat tgtagctatc atcatccatg atcttcgaaa gaccaaaatc tgtaattttt
      841 atctctccac acgctgtacc atttactaaa agaatattac ctggtttgag gtcatagtgt
      901 atgatgggag gttttatttc atttaagtac tttaaagcat tcacaatctg catgataatg
      961 gaccgggcct ctttctccga cattaatttg tgctgtttca gtagagtcca gatcatttcc
     1021 ctcccagtat tctaatactg tacaaacgag tcagtttcca gtgaaaagta tcatacagct
     1081 tactatttct gggatgatcc agcttttttt tgatcgnatt ccctaacatg catgctggng
     1141 nattctcctt ttttctccat ctctcttcca gttttttatt taactgggtg aattttcaca
     1201 ggcttacg
//
