LOCUS       sequence-260311-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK2-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(344..385)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    387..411
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggcgcctt attctgcatg caagcatccc cactcttcag ctcgccgatt cgatccagca
       61 ggtactgatt ggtggagtcg accacaggca ggactgccac gctcccgccg tccagctgtc
      121 ccagaatctg tttagcgttc agcagcggga taggctcggg cagagagtag ccctttccgg
      181 gcacggtgaa cacgtccacg ccccagtcgc gcagagtctg aatgtgcttg ttgatagcgg
      241 ccctggacat gcccagggtt tctcccagct gttcgccact atggaactcg ccattagcca
      301 ggagagcgat cagcttcaga ggcacagtat tgtctttgct agcggtgctg tccaggccca
      361 gcagggggtt ggggatgggc ttgccgccca ctttgtacaa gaaagctggg cccatgaggc
      421 ctgaactttt cgggataatt ccacatttgg aaagccgccc cgtcatcatt tccatacagt
      481 tagtggtgtc tgacaagtgc tgggccgcag gcgggtcgtg tgtgctcagc agctgggcct
      541 ggatggtctc caccaccctc ttgaagcgac ggctggggcc tgagagcagg gtgaaggtga
      601 cggagtagat gccgttctcc ttctgcgcct ccccaccctc cgtgtaggtg atatcaacct
      661 ggaacttgac cggcttctgg aacacggctg gcccccccgt ggccttgtac tcggcccgga
      721 agctcgtttg ggagatgacg ctgtggctga gactgggaat cgacaggaag gcgtgcacga
      781 tgtcagcctt gatggagctc agaggtttgt ctttgatgac cacgagatct gctcctcctt
      841 ctccaggctg atgagttccc aaaccaggac ttctcgccag ctctggggac gactctgggt
      901 gtcaagttgg acatctcctc cggcgtcgga acttgcagtt tccggcggtg gaaccggggt
      961 gagccca
//
