LOCUS       sequence-260313-        1245 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with C-terminal V5
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1245)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK2-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1245
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    134..158
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 antggctgcg atgtgttttg tgagtttttt aggcaccttt tgaatgtaat catttgggtc
       61 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
      121 ttttttgtta gacgcaactt tgtacaaaaa agcagaaggg ccgtcaaggc ccaccatgac
      181 atcgacgggg aaggacggcg gcgcgcagca cgcgcagtat gttgggccct accggctgga
      241 gaagacgctg ggcaaggggc agacaggtct ggtgaagctg ggggttcact gcgtcacctg
      301 ccagaaggtg gccatcaaga tcgtcaaccg tgagaagctc agcgagtcgg tgctgatgaa
      361 ggtggagcgg gagatcgcga tcctgaagct cattgagcac ccccacgtcc taaagctgca
      421 cgacgtttat gaaaacaaaa aatatttgta cctggtgcta gaacacgtgt caggtggtga
      481 gctcttcgac tacctggtga agaaggggag gctgacgcct aaggaggctc ggaagttctt
      541 ccggcagatc atctctgcgc tggacttctg ccacagccac tccatatgcc acagggatct
      601 gaaacctgaa aacctcctgc tggacgagaa gaacaacatc cgcatcgcag actttggcat
      661 ggcgtccctg caggttggcg acagcctgtt ggagaccagc tgtgggtccc cccactacgc
      721 ctgccccgag gtgatccggg gggagaagta tgacggccgg aaggcggacg tgtggagctg
      781 cggcgtcatc ctgttcgcct tgctggtggg ggctctgccc ttcgacgatg acaacttgcg
      841 acagctgctg gagaaaggtg aagcggggcg tgttccacat gccgcacttt atcccgcccg
      901 actgccagag tctgctacgg ggcatgatcg aggtggacgc cgcacgccgc ctcacgctag
      961 agcacattca gaaacacata tggtatatag ggggcaagaa tgagcccgaa ccagagcagc
     1021 ccattcctcg ccaggtgcag atccgctcgc tgcccagcct ggaggacatc gaccccgacg
     1081 tggctgggac agcatgcacc tcacttgggc tgcttcggag accgcaacaa gctgctgcag
     1141 actgctgtcg aggaggaaaa accaggaaaa gaatgattta ctnctcctcc tgggaacgga
     1201 nnaagnacgg nncggagaat gagaaaccgg tcccctcggg gaaac
//
