LOCUS       sequence-260314-        1183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with C-terminal V5
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1183)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK2-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..92
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     209..228
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(287..328)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    327..351
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tnttttaagt catctttcca attttgtaat ccagaggttg attgtcgagc ggccgccact
       61 gtgctggata tcaactatgt ataataaagt tgattacagg cgggcaagaa tgtgaataaa
      121 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
      181 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
      241 acgatgccat tgggatatat caacggtggt atatccagtg ttattacgta gaatcgagac
      301 cgaggagagg gttagggata ggcttgccca ctttgtacaa gaaagctggg cccatgaggc
      361 ctgaactttt cgggataatt ccacatttgg aaagccgccc cgtcatcatt tccatacagt
      421 tagtggtgtc tgacaagtgc tgggccgcag gcgggtcgtg tgtgctcagc agctgggcct
      481 ggatggtctc caccaccctc ttgaagcgac ggctggggcc tgagagcagg gtgaaggtga
      541 cggagtagat gccgttctcc ttctgcgcct ccccaccctc cgtgtaggtg atatcaacct
      601 ggaacttgac cggcttctgg aacacggctg gcccccccgt ggccttgtac tcggcccgga
      661 agctcgtttg ggagatgacg ctgtggctga gactgggaat cgacaggaag gcgtgcacga
      721 tgtcagcctt gatggagctc agaggtttgt ctttgatgac cacgaagatc tgctcctcct
      781 tctccaggct gatgaagttc ccaaaccagg acttcttcgc cagctctggg gacgactctg
      841 gtgtcacgtt ggacatctcc tccggcgtcg gaacttgcag tttccggcgg tggaagcggg
      901 gtgagctcag aaagctgttc ttgatggagt tgagccgcgc cctccagggc acctctccga
      961 cgctggggct ggacggggcg tggggttggg cgtgccagcc gggctctcct ttggcgtgtg
     1021 acagtgtccc ctggggggtg ggggagggga ctgcccttgt gagggtgagg ggtcatccgg
     1081 gggctgctga atggcctggt gaaagctgag aggcacgctg atggacgaaa ctctagccgt
     1141 gctgncatcn atgncgacgc caagcaccgg ngatcgtcaa ttc
//
