LOCUS       sequence-260315-       12061 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12061)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-AAK1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..12061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             2448..2628
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2672..2797
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3290..3523
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3707..3751
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4018..4134
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4166..4186
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4186..5397
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5234..5253
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5398..5422
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8045..8069)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8071..8112
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8119..8889
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(8895..8915)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    8944..9532
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8997..9017)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9415..9426)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9545..10044
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9580..9603)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9977..9996
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10065..10664
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10065..10084)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10561..10581
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10802..11035
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11107..11228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11144..11163)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11198..11217
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11247..11382
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11414..11433)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11415..11433)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11443..11460)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11443..11459)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11452..11474)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11604..12059
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11691..11710)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11901..11922
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
     2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
     2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
     3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
     4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4081 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgtc
     4141 cagtgtggtg gaattctgca gatatcaact ttgtatagaa aagttggcct ccgcgccggg
     4201 ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag
     4261 ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat
     4321 aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg
     4381 actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc
     4441 tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg
     4501 cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg
     4561 tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt
     4621 ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct
     4681 gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc
     4741 tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag
     4801 gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa
     4861 gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc
     4921 actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt
     4981 gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt
     5041 ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc
     5101 cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac
     5161 ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat
     5221 cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt
     5281 tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag
     5341 tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgca
     5401 actttgtaca aaaaagcagg ctccgaattc gcccttatga agaagttttt cgactcccgg
     5461 cgagagcagg gcggctctgg cctgggctcc ggctccagcg gaggaggggg cagcacctcg
     5521 ggcctgggca gtggctacat cggaagagtc ttcggcatcg ggcgacagca ggtcacagtg
     5581 gacgaggtgt tggcggaagg tggatttgct attgtatttc tggtgaggac aagcaatggg
     5641 atgaaatgtg ccttgaaacg catgtttgtc aacaatgagc atgatctcca ggtgtgcaag
     5701 agagaaatcc agataatgag ggatctttca gggcacaaga atattgtggg ttacattgat
     5761 tctagtatca acaacgtgag tagcggtgat gtatgggaag tgctcattct gatggacttt
     5821 tgtagaggtg gccaggtggt aaacctgatg aaccagcgcc tgcaaacagg ctttacagag
     5881 aatgaagtgc tccagatatt ttgtgatacc tgtgaagctg ttgcccgcct gcatcagtgc
     5941 aaaactccta ttatccaccg ggacctgaag gttgaaaaca tcctcttgca tgaccgaggc
     6001 cactatgtcc tgtgtgactt tggaagcgcc accaacaaat tccagaatcc acaaactgag
     6061 ggagtcaatg cagtagaaga tgagattaag aaatacacaa cgctgtccta tcgagcacca
     6121 gaaatggtca acctgtacag tggcaaaatc atcactacga aggcagacat ttgggctctt
     6181 ggatgtttgt tgtataaatt atgctacttc actttgccat ttggggaaag tcaggtggca
     6241 atttgtgatg gaaacttcac aattcctgat aattctcgat attctcaaga catgcactgc
     6301 ctaattaggt atatgttgga accagaccct gacaaaaggc cggatattta ccaggtgtcc
     6361 tacttctcat ttaagctact caagaaagag tgcccaattc caaatgtaca gaactctccc
     6421 attcctgcaa agcttcctga accagtgaaa gccagtgagg cagctgcaaa aaagacccag
     6481 ccaaaggcca gactgacaga tcccattccc accacagaga cttcaattgc accccgccag
     6541 aggcctaaag ctgggcagac tcagccgaac ccaggaatcc ttcccatcca gccagcgctg
     6601 acaccccgga agagggccac tgttcagccc ccacctcagg ctgcaggatc cagcaatcag
     6661 cctggccttt tagccagtgt tccccaacca aaaccccaag ccccacccag ccagcctctg
     6721 ccgcaaactc aggccaagca gccacaggct cctcccactc cacagcagac gccttctact
     6781 caggcccagg gtctgcccgc tcaggcccag gccacacccc agcaccagca gcaactcttc
     6841 ctcaagcagc aacagcagca gcaacagcca ccgccagcac agcagcagcc ggcaggcacg
     6901 ttttaccagc agcagcaggc ccagactcag cagtttcagg cagtacatcc agcaacccag
     6961 aaaccagcaa ttgctcagtt ccctgtggtg tcccaaggag gctctcaaca gcagctaatg
     7021 cagaatttct accagcagca gcagcagcag caacaacaac agcaacagca acagctggcc
     7081 acagccctgc atcaacaaca gctgatgact cagcaggctg ccttgcagca aaagcccact
     7141 atggcagcag gacagcagcc ccagccacag ccagctgcag ccccacagcc agcccctgcc
     7201 caggagccag cgattcaagc cccagtaaga caacagccaa aggttcagac aaccccacct
     7261 cctgccgtcc aggggcagaa agttggatct ctcactccac cctcatcccc caaaacccaa
     7321 cgtgctgggc acaggcgtat tctcagtgac gtaacccaca gtgcagtctt tggggtccct
     7381 gccagcaaat caacccagct gctccaggca gctgcagctg aggccagtct caataagtcc
     7441 aagtctgcaa ccaccactcc atcaggctct cctcggacct ctcaacaaaa cgtttataat
     7501 ccttcagaag ggtctacgtg gaatcccttt gatgacgata atttctccaa actcacagct
     7561 gaagaactgc taaacaagga ctttgccaag cttggggaag gcaaacatcc cgagaagctt
     7621 ggaggctcag ctgagagttt gatcccaggc tttcaatcaa cccaaggtga tgcttttgct
     7681 acgacctcat tttctgctgg aactgctgaa aaaaggaagg gtgggcagac tgtggactct
     7741 ggcctcccgc ttctaagcgt gtctgatcct ttcattcctc ttcaagtacc tgatgcacca
     7801 gaaaaactaa ttgagggact caagtctcct gacacttctc ttctgctccc tgacctcttg
     7861 cctatgacag atccttttgg tagcacttct gatgctgtaa ttggtaaagt catcatctct
     7921 gtttcttcag tcatgcatga tatgtgtgcc tgtttcaaga atgacaagta cctagttaac
     7981 caatccctgg ggaatagccc tgccacccca gaagccaagg ctattgaatt caagggcgaa
     8041 ttcgacccag ctttcttgta caaagtgggc ggcaagccca tccccaaccc cctgctgggc
     8101 ctggacagca ccgctagcat cccgctgctg aacgctaaac agattctggg acagctggac
     8161 ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct gctggatcga
     8221 atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc agggagagga
     8281 agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag tatgttttgg
     8341 cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat tgtcatggca
     8401 gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa tgacctgtat
     8461 ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac aggcgatgct
     8521 gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga ggaaagcgtg
     8581 gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag gaatactctg
     8641 gccgctatgc tgatccgaga gctgcgggca gccctggaac tgttcgagca ggaaggcctg
     8701 gctccatatc tgtcacggtg ggagaagctg gataacttca tcaatagacc cgtgaagctg
     8761 atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca gggagccctg
     8821 ctgctggaac aggacggagt tatcaaaccc tggatgggcg gagaaatcag tctgcggtct
     8881 gccgaaaagt aagccaactt tattatacat agttgatatc cagcacagtg gcggccgctc
     8941 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     9001 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     9061 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     9121 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     9181 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     9241 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     9301 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     9361 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     9421 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     9481 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     9541 caccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     9601 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     9661 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     9721 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     9781 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     9841 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     9901 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     9961 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
    10021 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
    10081 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
    10141 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
    10201 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
    10261 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
    10321 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
    10381 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
    10441 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
    10501 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
    10561 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
    10621 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
    10681 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta
    10741 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
    10801 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
    10861 ctctggttag accagatctg agcctgggag ctctctggct gcctagggaa cccactgctt
    10921 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10981 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
    11041 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
    11101 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    11161 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    11221 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt
    11281 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
    11341 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga
    11401 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
    11461 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
    11521 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
    11581 cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
    11641 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
    11701 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    11761 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    11821 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    11881 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    11941 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
    12001 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc
    12061 c
//
