LOCUS       sequence-260316-        1183 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1183)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-AAK1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1183
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    138..162
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggannggctg cgagtgtgtt ttgtgagttt tttaggcacc ttttgaaatg taatcatttg
       61 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt
      121 tggctttttt gttagacgca actttgtaca aaaaagcagg ctccgaattc gcccttatga
      181 agaagttttt cgactcccgg cgagagcagg gcggctctgg cctgggctcc ggctccagcg
      241 gaggaggggg cagcacctcg ggcctgggca gtggctacat cggaagagtc ttcggcatcg
      301 ggcgacagca ggtcacagtg gacgaggtgt tggcggaagg tggatttgct attgtatttc
      361 tggtgaggac aagcaatggg atgaaatgtg ccttgaaacg catgtttgtc aacaatgagc
      421 atgatctcca ggtgtgcaag agagaaatcc agataatgag ggatctttca gggcacaaga
      481 atattgtggg ttacattgat tctagtatca acaacgtgag tagcggtgat gtatgggaag
      541 tgctcattct gatggacttt tgtagaggtg gccaggtggt aaacctgatg aaccagcgcc
      601 tgcaaacagg ctttacagag aatgaagtgc tccagatatt ttgtgatacc tgtgaagctg
      661 ttgcccgcct gcatcagtgc aaaactccta ttatccaccg ggacctgaag gttgaaaaca
      721 tcctcttgca tgaccgaggc cactatgtcc tgtgtgactt tggaagcgcc accaacaaat
      781 tccagaatcc acaaactgag ggagtcaatg cagtagaaga tgagattaag aaatacacaa
      841 cgctgtccta tcgagcacca gaaatggtca acctgtacag tggcaaaatc atcactacga
      901 agcagacatt tgggctcttg gatgtttgtt gtataaatta tgctacttca ctttgccatt
      961 tggggaaagt caggtggcaa tttgtgatgg aaacttcaca ttcctgatat tctcgatatt
     1021 tctcagacat gcactgctat tagtattatg ttggaaccag accctgacaa agcgatattt
     1081 acaggtgtct actctcattt agctactcag aaagangcca tcaaatgtac gactctccat
     1141 cctgccaagc ttctgacagt gaagcccagg ggaggcagct gcc
//
