LOCUS       sequence-260317-        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-AAK1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(156..197)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    199..223
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggggngtcga ttctgcatgc agcatcccca ctcttcagct cgccgattcg atccagcagg
       61 tactgattgg tggagtcgac cacaggcagg actgccacgc tcccgccgtc cagctgtccc
      121 agaatctgtt tagcgttcag cagcgggatg ctagcggtgc tgtccaggcc cagcaggggg
      181 ttggggatgg gcttgccgcc cactttgtac aagaaagctg ggtcgaattc gcccttgaat
      241 tcaatagcct tggcttctgg ggtggcaggg ctattcccca gggattggtt aactaggtac
      301 ttgtcattct tgaaacaggc acacatatca tgcatgactg aagaaacaga gatgatgact
      361 ttaccaatta cagcatcaga agtgctacca aaaggatctg tcataggcaa gaggtcaggg
      421 agcagaagag aagtgtcagg agacttgagt ccctcaatta gtttttctgg tgcatcaggt
      481 acttgaagag gaatgaaagg atcagacacg cttagaagcg ggaggccaga gtccacagtc
      541 tgcccaccct tccttttttc agcagttcca gcagaaaatg aggtcgtagc aaaagcatca
      601 ccttgggttg attgaaagcc tgggatcaaa ctctcagctg agcctccaag cttctcggga
      661 tgtttgcctt ccccaagctt ggcaaagtcc ttgtttagca gttcttcagc tgtgagtttg
      721 gagaaattat cgtcatcaaa gggattccac gtagaccctt ctgaaggatt ataaacgttt
      781 tgttgagagg tccgaggaga gcctgatgga gtagtggttg cagacttgga cctattgaga
      841 ctggcctcag ctgcagctgc ctggagcagc tgggttgatt tgctggcagg accccaaaga
      901 ctgcactgtg ggttacgtca ctgagaatac gcctgtgccc agcacgttgg gtttggggga
      961 tgagggtgga gtgagagatc acttctgccc tgaacgcagg agtggggttg tctgaacctt
     1021 aggctgatgc ctcacggtgc ttgatcccat ggatcc
//
