LOCUS       sequence-260319-        1164 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1164)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-AAK1-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..1164
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    135..159
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccggctggcg agtgtgtttt gtgagttttt taggcacctt ttgaatgtaa tcatttgggt
       61 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
      121 cttttttgtt agacgcaact ttgtacaaaa aagcaggctc cgaattcgcc cttatgaaga
      181 agtttttcga ctcccggcga gagcagggcg gctctggcct gggctccggc tccagcggag
      241 gagggggcag cacctcgggc ctgggcagtg gctacatcgg aagagtcttc ggcatcgggc
      301 gacagcaggt cacagtggac gaggtgttgg cggaaggtgg atttgctatt gtatttctgg
      361 tgaggacaag caatgggatg aaatgtgcct tgaaacgcat gtttgtcaac aatgagcatg
      421 atctccaggt gtgcaagaga gaaatccaga taatgaggga tctttcaggg cacaagaata
      481 ttgtgggtta cattgattct agtatcaaca acgtgagtag cggtgatgta tgggaagtgc
      541 tcattctgat ggacttttgt agaggtggcc aggtggtaaa cctgatgaac cagcgcctgc
      601 aaacaggctt tacagagaat gaagtgctcc agatattttg tgatacctgt gaagctgttg
      661 cccgcctgca tcagtgcaaa actcctatta tccaccggga cctgaaggtt gaaaacatcc
      721 tcttgcatga ccgaggccac tatgtcctgt gtgactttgg aagcgccacc aacaaattcc
      781 agaatccaca aactgaggga gtcaatgcag tagaagatga gattaagaaa tacacaacgc
      841 tgtcctatcg agcaccagaa atggtcaacc tgtacagtgg caaaatcatc actacgaacg
      901 cagacatttg ggctcttgga tgtttgttgt ataaattatg ctacttcact ttgccatttg
      961 gggaaagtca gtggcaattt gtgatggaaa cttcacattc ctgatattct cgatattctc
     1021 agacatgcac tgcctaatta ggtatatgtt gggaccagac cctgacaaag ccgatattac
     1081 cagtgtccta cttctcattt agctactcag aagangccaa ttccaaatgt acagaactcc
     1141 tcccattcct ggcaagcttc ctga
//
