LOCUS       sequence-260320-        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-AAK1-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(341..382)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    384..408
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggncgtctat tctgcatgca gcatccccac tcttcagctc gccgattcga tccagcaggt
       61 actgattggt ggagtcgacc acaggcagga ctgccacgct cccgccgtcc agctgtccca
      121 gaatctgttt agcgttcagc agcgggatag gctcgggcag agagtagccc tttccgggca
      181 cggtgaacac gtccacgccc cagtcgcgca gagtctgaat gtgcttgttg atagcggccc
      241 tggacatgcc cagggtttct cccagctgtt cgccactatg gaactcgcca ttagccagga
      301 gagcgatcag cttcagaggc acagtattgt ctttgctagc ggtgctgtcc aggcccagca
      361 gggggttggg gatgggcttg ccgcccactt tgtacaagaa agctgggtcg aattcgccct
      421 tgaattcaat agccttggct tctggggtgg cagggctatt ccccagggat tggttaacta
      481 ggtacttgtc attcttgaaa caggcacaca tatcatgcat gactgaagaa acagagatga
      541 tgactttacc aattacagca tcagaagtgc taccaaaagg atctgtcata ggcaagaggt
      601 cagggagcag aagagaagtg tcaggagact tgagtccctc aattagtttt tctggtgcat
      661 caggtacttg aagaggaatg aaaggatcag acacgcttag aagcgggagg ccagagtcca
      721 cagtctgccc acccttcctt ttttcagcag ttccagcaga aaatgaggtc gtagcaaaag
      781 catcaccttg ggttgattga aagcctggga tcaaactctc agctgagcct ccaagcttct
      841 cgggatgttt gccttcccca agcttggcaa agtccttgtt tagcagttct tcagctgtga
      901 gtttggagaa ttatcgtcat caaagggatt ccacgtagac ccttctgaag gattataaac
      961 gttttgttga gagtccgagg agagcctgat ggagtagtgg tttgcagact tgacctattg
     1021 agaactgggc ctcagctgca gctgcctgga gcagctgggt tgatttgctg gcagggaccc
     1081 gaagactgca ctggtgggtt acgtcactga aaatacgcct gtgccagcac gttgggtttt
     1141 ggggggatg
//
