LOCUS       sequence-260322-        1211 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with C-terminal V5 
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1211)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-AAK1-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1211
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    134..158
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atggatggcg gtgtgttttg tgagtttttt aggcaccttt tgaatgtaat catttgggtc
       61 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
      121 ttttttgtta gacgcaactt tgtacaaaaa agcaggctcc gaattcgccc ttatgaagaa
      181 gtttttcgac tcccggcgag agcagggcgg ctctggcctg ggctccggct ccagcggagg
      241 agggggcagc acctcgggcc tgggcagtgg ctacatcgga agagtcttcg gcatcgggcg
      301 acagcaggtc acagtggacg aggtgttggc ggaaggtgga tttgctattg tatttctggt
      361 gaggacaagc aatgggatga aatgtgcctt gaaacgcatg tttgtcaaca atgagcatga
      421 tctccaggtg tgcaagagag aaatccagat aatgagggat ctttcagggc acaagaatat
      481 tgtgggttac attgattcta gtatcaacaa cgtgagtagc ggtgatgtat gggaagtgct
      541 cattctgatg gacttttgta gaggtggcca ggtggtaaac ctgatgaacc agcgcctgca
      601 aacaggcttt acagagaatg aagtgctcca gatattttgt gatacctgtg aagctgttgc
      661 ccgcctgcat cagtgcaaaa ctcctattat ccaccgggac ctgaaggttg aaaacatcct
      721 cttgcatgac cgaggccact atgtcctgtg tgactttgga agcgccacca acaaattcca
      781 gaatccacaa actgagggag tcaatgcagt agaagatgag attaagaaat acacaacgct
      841 gtcctatcga gcaccagaaa tggtcaacct gtacagtggc aaaatcatca ctacgaaagg
      901 cagacatttg ggctcttgga tgtttgttgt ataaattatg ctacttcact ttgccatttg
      961 gggaaagtca gtggcaattt gtgatgggaa acttcacaat tcctgataat tctcgatatt
     1021 ctcagacatg cactgcctaa ttaggtatta tgttggaacc agaccctgac aaagcggata
     1081 ttacaggtgt cctacttcct cattagctac tcagaaagag ntgcccattc caaatgtang
     1141 antctccaat cctgcagcct ctgancagtg aagcagtgag cagctgcaaa aaagaatcca
     1201 gccaaggtca g
//
