LOCUS       sequence-260323-        1235 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with C-terminal V5 
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1235)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-AAK1-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..1235
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..93
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     210..229
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             complement(288..329)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    328..352
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gnnttcagct catctttcca aattttgtaa tccagaggtt gattgtcgag cggccgccac
       61 tgtgctggat atcaactatg tataataaag ttgattacag gcgggcaaga atgtgaataa
      121 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      181 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      241 tacgatgcca ttgggatata tcaacggtgg tatatccagt gttattacgt agaatcgaga
      301 ccgaggagag ggttagggat aggcttgccc actttgtaca agaaagctgg gtcgaattcg
      361 cccttgaatt caatagcctt ggcttctggg gtggcagggc tattccccag ggattggtta
      421 actaggtact tgtcattctt gaaacaggca cacatatcat gcatgactga agaaacagag
      481 atgatgactt taccaattac agcatcagaa gtgctaccaa aaggatctgt cataggcaag
      541 aggtcaggga gcagaagaga agtgtcagga gacttgagtc cctcaattag tttttctggt
      601 gcatcaggta cttgaagagg aatgaaagga tcagacacgc ttagaagcgg gaggccagag
      661 tccacagtct gcccaccctt ccttttttca gcagttccag cagaaaatga ggtcgtagca
      721 aaagcatcac cttgggttga ttgaaagcct gggatcaaac tctcagctga gcctccaagc
      781 ttctcgggat gtttgccttc cccagcttgg caaagtcctt gtttagcagt tcttcagctg
      841 tgagtttgga gaattatcgt catcaaaggg attccacgta gacccttctg aggattataa
      901 cgtttgttga aggtccgagg agagcctgat gagtagtgtt gcagactgga cctattgaga
      961 ctggcctcag ctgcagctgc tgagcagctg ggttgatttg ctgcaggacc caaagactgc
     1021 actgtggtta cgtcactgaa nacgcctgtg cccagcacgt ngntggggat tgagggtgga
     1081 atgaaaaatc ccacttctcc cttgacggca gaggtggttg tctgaacttg gtgtgtccta
     1141 ctgnntggaa tcttcgnnng caaggactta gccttaggtg tccactgatt ggctggnncg
     1201 tcctgtgcaa ntggnctttg ctgcagacnc tgcat
//
