LOCUS       sequence-260328-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK1 (human) tagged with C-terminal V5
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK1-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    80..100
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     217..236
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    335..359
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctnaaatata aaaattcatc tttcaaatat tttgtactcc agatgttgag tgtcgagcgg
       61 ccgccactgt gctggatatc aactatgtat aataaagttg attacaggcg ggcaagaatg
      121 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
      181 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
      241 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgtt attacgtaaa
      301 atcaagaccg aggagagggt tagggatagg cttgcccact ttgtacaaga aagctgggtt
      361 gggcaaaggg gtcccgttgg tggccaggag cttcttgtcc ttgggggggc ctcggcgggg
      421 agagctgctc agctctgggt ctgggcggcc gggtgggggc tgcaggcttc ggggtggggc
      481 accagcaggc cgggtctggg ccccgttctt ctcgtctgcc agggcctgca cggagggctg
      541 gtcatgagtg ctcaggagct gtgcctggat ggtctccacc actcgcttga accgacggct
      601 gggacccgag atgagagtga aggtgacgga gtagatgcca ccacctccgc tgccgtcccg
      661 tcgcggggag ggctctggac cctcagagga actgatgtcc acctggaagc ggacgggctt
      721 ttggaatacg gaggggccgc cactgggcct tgtactcggc ccctgaagct ggtctgtgac
      781 agcacactgt gactcatgct ggggatcgac aaaaaggcat ggacgatgtc tgctttgatg
      841 ctgctggaag gtttgtcctt tagcaccagg aatattttgt tcttctttgt ccaaggagat
      901 gaagtttccc gaaccaagag cgtttttggc aagctcccgg agaaggactc tgggcgtcaa
      961 a
//
