LOCUS       sequence-260331-        1179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of dsRed (as control) tagged with 
            N-terminal miniTurbo-V5 for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1179)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-dsRed_IDG-K
FEATURES             Location/Qualifiers
     source          1..1179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(141..161)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      162..171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    986..1010
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gnngccgtgg tgcggtgtgt tttgtgagtt ttttaggcac cttttgaatg taatcatttg
       61 ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt
      121 tggctttttt gttagacggc caacttttgt atacaaagtg ggccaccatg ggaatcccgc
      181 tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca gtcctgcctg
      241 tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag agtggggatg
      301 cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa tggttctctc
      361 cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa
      421 tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag ctgggagcag
      481 acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag ctggcaggca
      541 tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt ggcgccggga
      601 ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc
      661 aggaagcagg gattaacctg gacaggaata ctctggccgc tatgctgatc cgagagctgc
      721 gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgtca cggtgggaga
      781 agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa gagattttcg
      841 ggattagccg ggggattgat aaacaggagc cctgctgctg gaacaggacg gagttatcaa
      901 accctggatg ggcggagaaa tcagtctgcg gtctgccgaa agctagcggc aagcccatcc
      961 cacccctgct gggctggaca gcaccacaac tttgtacaaa aaagttgcac catggatagc
     1021 actgagaacg tcatcagccc ttcatgcgct tcaggtgcca catggaggct cgngacgcca
     1081 cgagttcnag atcgaggcga ggtagtcagg cttacgaggc acnaaccgca gctgcaggac
     1141 aagggcgccc ctgccttcnc tggacatcct gtccccaat
//
