LOCUS       sequence-260332-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of dsRed (as control) tagged with 
            N-terminal miniTurbo-V5 for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-dsRed_IDG-K
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             125..166
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    167..191
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(374..394)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     592..611
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gggngcaaga tttcgggatt agccggggga tgataaacag ggagccctgc tgctggaaca
       61 ggacggagtt atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg ccgaaaaggc
      121 tagcggcaag cccatcccca accccctgct gggcctggac agcaccacaa ctttgtacaa
      181 aaaagttggc accatggata gcactgagaa cgtcatcaag cccttcatgc gcttcaaggt
      241 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggcaa
      301 gccctacgag ggcacccaga ccgccaagct gcaggtgacc aagggcggcc ccctgccctt
      361 cgcctgggac atcctgtccc cccagttcca gtacggctcc aaggtgtacg tgaagcaccc
      421 cgccgacatc cccgactaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      481 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
      541 caccttcatc taccacgtga agttcatcgg cgtgaacttc ccctccgacg gccccgtaat
      601 gcagaagaag actctgggct gggagccctc caccgagcgc ctgtaccccc gcgacggcgt
      661 gctgaagggc gagatccaca aggcgctgaa gctgaagggc ggcggccact acctggtgga
      721 gttcaagtca atctacatgg ccaagaagcc cgtgaagctg cccggctact actacgtgga
      781 ctccaagctg gacatcacct cccacacgag gactacaccg tggtggagca gtacgagcgc
      841 gccgaggccc gccaccacct gttccagtaa tcactttctt gtacaagttg tgatatccag
      901 cacagtggcg ccgctcgaca tcactctgga tacaaatttg tgaagatgac tggtattcta
      961 ctatgtgctc tttacgctat gtgatacgct gctangcttg atcatgctat tgctcccgtt
     1021 gcctcattcc cctcctgaaa atctgtgctg tctctttaga gattgggccc gtgtcagcac
     1081 tggctgggg
//
