LOCUS       sequence-260334-        1232 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of dsRed (as control) tagged with 
            N-terminal TurboID-V5 for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1232)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-TurboID-V5-dsRed_IDG-K
FEATURES             Location/Qualifiers
     source          1..1232
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(139..159)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      160..169
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gnntgctgcg agtgtgtttt gtgagttttt taggcacctt ttgaaatgta atcatttggg
       61 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
      121 gcttttttgt tagacggcca acttttgtat acaaagtggg ccaccatggg aaaagacaat
      181 actgtgcctc tgaagctgat cgctctcctg gctaatggcg agttccatag tggcgaacag
      241 ctgggagaaa ccctgggcat gtccagggcc gctatcaaca agcacattca gactctgcgc
      301 gactggggcg tggacgtgtt caccgtgccc ggaaagggct actctctgcc cgagcctatc
      361 ccgctgctga acgctaaaca gattctggga cagctggacg gcgggagcgt ggcagtcctg
      421 cctgtggtcg actccaccaa tcagtacctg ctggatcgaa tcggcgagct gaagagtggg
      481 gatgcttgca ttgcagaata tcagcaggca gggagaggaa gcagagggag gaaatggttc
      541 tctccttttg gagctaacct gtacctgagt atgttttggc gcctgaagcg gggaccagca
      601 gcaatcggcc tgggcccggt catcggaatt gtcatggcag aagcgctgcg aaagctggga
      661 gcagacaagg tgcgagtcaa atggcccaat gacctgtatc tgcaggatag aaagctggca
      721 ggcatcctgg tggagctggc cggaataaca ggcgatgctg cacagatcgt cattggcgcc
      781 gggattaacg tggctatgag gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca
      841 ctgcacgaag caggattaac ctggacagga atactctggc cgctacgctg atccgagagc
      901 tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg ccacgtggga
      961 gaagctggat aacttcatca atagacccgt gaagctgatc attgggacaa gagattttcg
     1021 ggattagccg gggaattgat aaacaggagc cctgctgctg ggacagacga ttatcaacct
     1081 gatgggcgga gaatcaatct gcgctgccga aaagctagcg caagccatcc acccctgctt
     1141 gctgaaccgc atccacttgt tcaaaaagtg caccatgnat gcngaacgta ctcagccntc
     1201 atggccctca aggttnacat tgaggctcct cg
//
