LOCUS       sequence-260335-        1161 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of dsRed (as control) tagged with 
            N-terminal TurboID-V5 for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1161)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-TurboID-V5-dsRed_IDG-K
FEATURES             Location/Qualifiers
     source          1..1161
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             127..168
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             196..873
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(376..396)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     594..613
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     788..811
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(872..896)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(979..999)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ggtcggagag atttcggcat tagccggggg attgataaac agggagccct gctgctggaa
       61 caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag
      121 gctagcggca agcccatccc caaccccctg ctgggcctgg acagcaccac aactttgtac
      181 aaaaaagttg gcaccatgga tagcactgag aacgtcatca agcccttcat gcgcttcaag
      241 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      301 aagccctacg agggcaccca gaccgccaag ctgcaggtga ccaagggcgg ccccctgccc
      361 ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta cgtgaagcac
      421 cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa gtgggagcgc
      481 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      541 ggcaccttca tctaccacgt gaagttcatc ggcgtgaact tcccctccga cggccccgta
      601 atgcagaaga agactctggg ctgggagccc tccaccgagc gcctgtaccc ccgcgacggc
      661 gtgctgaagg gcgagatcca caaggcgctg aagctgaagg gcggcggcca ctacctggtg
      721 gagttcaagt caatctacat ggccaagaag cccgtgaagc tgcccggcta ctactacgtg
      781 gactccaagc tggacatcac ctcccacaac gaggactaca ccgtggtgga gcagtacgag
      841 cgcgccgagg cccgccacca cctgttccag taatcaactt tcttgtacaa agttgtgata
      901 tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt
      961 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     1021 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     1081 gctgctgtct ctttatgaga gttgtggccc gttgtcagca acgnggcggt ggnggtgcac
     1141 tggtgtttgc tgacgccann c
//
