LOCUS       sequence-260337-        1171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of dsRed (as control) tagged with 
            N-terminal V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1171)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-dsRed_IDG-K
FEATURES             Location/Qualifiers
     source          1..1171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(140..160)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             164..432
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             442..483
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    481..505
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(688..708)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     905..924
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gnnnctgctg cggtgtgttt tgtgagtttt ttaggcacct tttgaaatgt aatcatttgg
       61 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt
      121 ggcttttttg ttagacggcc aacttttgta tacaaagtgg cgaggggctc gcatctctcc
      181 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
      241 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
      301 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
      361 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
      421 ccgttacaga tcggcaccat gggtaagcct atccctaacc ctctcctcgg tctcgattct
      481 acaactttgt acaaaaaagt tggcaccatg gatagcactg agaacgtcat caagcccttc
      541 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      601 gagggcgagg gcaagcccta cgagggcacc cagaccgcca agctgcaggt gaccaagggc
      661 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg
      721 tacgtgaagc accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc
      781 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      841 tccctgcaga cggcaccttc atctaccacg tgaagttcat cggcgtgaac ttcccctccg
      901 acggccccgt aatgcagaag aagactctgg gctgggagcc ctccaccgag cgcctgtacc
      961 cccgcgacgg cgtgctgaag gcgagatcca caaggcgctg aagctgaagg cggcggccac
     1021 tacctggtgg agttcaagtc aatctacatg gccagaagcc cgtgaagctg cccggctact
     1081 actacgtgac tcagctggac atcacctccc acacgagact aacaccggtg gtggagcagt
     1141 acgagcgcgc ccgaggcccg ccaccanctg t
//
