LOCUS       sequence-260338-        1130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of dsRed (as control) tagged with 
            N-terminal V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1130)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-dsRed_IDG-K
FEATURES             Location/Qualifiers
     source          1..1130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(95..772)
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(157..180)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(355..374)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     572..592
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     protein_bind    complement(775..799)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(797..838)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tnnatcagtc atctttccaa ttttgtaatc cagaggttga ttgtcgagcg gccgccactg
       61 tgctggatat cacaactttg tacaagaaag ttgattactg gaacaggtgg tggcgggcct
      121 cggcgcgctc gtactgctcc accacggtgt agtcctcgtt gtgggaggtg atgtccagct
      181 tggagtccac gtagtagtag ccgggcagct tcacgggctt cttggccatg tagattgact
      241 tgaactccac caggtagtgg ccgccgccct tcagcttcag cgccttgtgg atctcgccct
      301 tcagcacgcc gtcgcggggg tacaggcgct cggtggaggg ctcccagccc agagtcttct
      361 tctgcattac ggggccgtcg gaggggaagt tcacgccgat gaacttcacg tggtagatga
      421 aggtgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt
      481 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcttgtag tcggggatgt
      541 cggcggggtg cttcacgtac accttggagc cgtactggaa ctggggggac aggatgtccc
      601 aggcgaaggg cagggggccg cccttggtca cctgcagctt ggcggtctgg gtgccctcgt
      661 agggcttgcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca
      721 tgtgcacctt gaagcgcatg aagggcttga tgacgttctc agtgctatcc atggtgccaa
      781 cttttttgta caaagttgta gaatcgagac cgaggagagg gttagggata ggcttaccca
      841 tggtgccgat ctgtaacggc gcagaacaga aaacgaaaca aagacgtaga gttgagcaag
      901 cagggtcagg caaagcgtga gagccggctg agtctaggta ggctccaagg agcgccggac
      961 aaagcccggt ctcgacctga gctttaactt acctagacgc gacgcagttc agagcaccac
     1021 aggcggaggc gcagacgcga ctcacggcgt gatgcgctca gtagcgcggc gcgtgagaaa
     1081 gatgcagccc tcgccacttt gtatacaaag ttggccgtct aacaaaaagc
//
