LOCUS       sequence-260340-        1122 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of eGFP (as control) tagged with 
            N-terminal miniTurbo-V5 for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1122)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-eGFP_IDG-K
FEATURES             Location/Qualifiers
     source          1..1122
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(140..160)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      161..170
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    985..1009
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccggatggc gagtgtgttt tgtgagtttt ttaggcacct tttgaaatgt aatcatttgg
       61 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt
      121 ggcttttttg ttagacggcc aacttttgta tacaaagtgg gccaccatgg gaatcccgct
      181 gctgaacgct aaacagattc tgggacagct ggacggcggg agcgtggcag tcctgcctgt
      241 ggtcgactcc accaatcagt acctgctgga tcgaatcggc gagctgaaga gtggggatgc
      301 ttgcattgca gaatatcagc aggcagggag aggaagcaga gggaggaaat ggttctctcc
      361 ttttggagct aacctgtacc tgagtatgtt ttggcgcctg aagcggggac cagcagcaat
      421 cggcctgggc ccggtcatcg gaattgtcat ggcagaagcg ctgcgaaagc tgggagcaga
      481 caaggtgcga gtcaaatggc ccaatgacct gtatctgcag gatagaaagc tggcaggcat
      541 cctggtggag ctggccggaa taacaggcga tgctgcacag atcgtcattg gcgccgggat
      601 taacgtggct atgaggcgcg tggaggaaag cgtggtcaat cagggctgga tcacactgca
      661 ggaagcaggg attaacctgg acaggaatac tctggccgct atgctgatcc gagagctgcg
      721 ggcagccctg gaactgttcg agcaggaagg cctggctcca tatctgtcac ggtgggagaa
      781 gctggataac ttcatcaata gacccgtgaa gctgatcatt ggggacaaag agattttcgg
      841 gattagccgg gggattgata aacaggagcc ctgctgctgg aacaggacgg agttatcaaa
      901 ccctggatgg gcggagaatc agtctgcggt ctgccgaaaa gctagcggca agcccatccc
      961 caacccctgc tgggctgaca gcacacaact ttgtacaaaa aagttggcgc cacatggtga
     1021 gcaaggcgga gagctgtcac gggtggtgcc catctgncga gctgacgcga cgtaacgcca
     1081 caagttcagc gtgttccggc gagggcgaag ggcgatgcaa cc
//
