LOCUS       sequence-260343-        1148 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of eGFP (as control) tagged with 
            N-terminal TurboID-V5 for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1148)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-TurboID-V5-eGFP_IDG-K
FEATURES             Location/Qualifiers
     source          1..1148
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(137..157)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gggcgtggga tgtgtttttg tgagtttttt aggcaccttt tgaatgtaat catttgggtc
       61 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
      121 ttttttgtta gacggccaac ttttgtatac aaagtgggcc accatgggaa aagacaatac
      181 tgtgcctctg aagctgatcg ctctcctggc taatggcgag ttccatagtg gcgaacagct
      241 gggagaaacc ctgggcatgt ccagggccgc tatcaacaag cacattcaga ctctgcgcga
      301 ctggggcgtg gacgtgttca ccgtgcccgg aaagggctac tctctgcccg agcctatccc
      361 gctgctgaac gctaaacaga ttctgggaca gctggacggc gggagcgtgg cagtcctgcc
      421 tgtggtcgac tccaccaatc agtacctgct ggatcgaatc ggcgagctga agagtgggga
      481 tgcttgcatt gcagaatatc agcaggcagg gagaggaagc agagggagga aatggttctc
      541 tccttttgga gctaacctgt acctgagtat gttttggcgc ctgaagcggg gaccagcagc
      601 aatcggcctg ggcccggtca tcggaattgt catggcagaa gcgctgcgaa agctgggagc
      661 agacaaggtg cgagtcaaat ggcccaatga cctgtatctg caggatagaa agctggcagg
      721 catcctggtg gagctggccg gaataacagg cgatgctgca cagatcgtca ttggcgccgg
      781 gattaacgtg gctatgaggc gcgtggagga aagcgtggtc aatcagggct ggatcacact
      841 gcagaagcag gattaacctg gacaggaata ctctggccgc tacgctgatc cgagagctgc
      901 gggcagccct ggaactgttc gagcagaatg cctggctcca tatctgccac ggtgggagag
      961 ctggataact tcatcaataa gacccgtgaa gctgatcatt ggggacaaga gattttcggg
     1021 attagccggg gattgataaa cagggagccc tgctgctgac aggacggagt tatcaaccct
     1081 gatggcggag aaatcagtct ggcggtctgc gaaaaggcta gcggcaagcc aattccccaa
     1141 ccccctgc
//
