LOCUS       sequence-260347-        1088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of eGFP (as control) tagged with 
            N-terminal V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1088)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-eGFP_IDG-K
FEATURES             Location/Qualifiers
     source          1..1088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(140..161)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     489..508
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     747..768
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      810..819
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(842..883)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tntatcagtc atctttccaa ttttgtaatc cagaggttga ttgtcgagcg gccgccactg
       61 tgctggatat cacaactttg tacaagaaag ttgattactt gtacagctcg tccatgccga
      121 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
      181 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
      241 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
      301 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
      361 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
      421 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
      481 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
      541 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
      601 agcactgcac gccgtagtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      661 ggtggtgcag atgaacttca gggtcagctt gccgtagtgg catcgccctc gccctcgccc
      721 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      781 cccggtgaac agctcctcgc ccttgctcac catggtggcg ccaacttttt ttgtacaaag
      841 ttgtagaatc gagaccgagg agagggttag ggataggctt acccatggtg ccgatctgta
      901 cggcgcagaa cagaaacgaa acaaagacgt agagttgagc agcaggtcag caagcgtgaa
      961 agccgctgat ctagtagctc caagggagcg ccgacaggcc cgtcttcgac tgactttaaa
     1021 cttacctaga cggcggactc agttcagagg cacccacagg cgggaggcgg cagacgcgac
     1081 tctcaccg
//
