LOCUS       sequence-260358-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-BRSK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(137..157)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      158..167
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             170..940
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             947..988
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    989..1013
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctggctgcgg tgtgttttgt gagtttttta ggcacctttt gaaatgtaat catttgggtc
       61 aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc
      121 ttttttgtta gacggccaac ttttgtatac aaagtgggcc accatgggaa tcccgctgct
      181 gaacgctaaa cagattctgg gacagctgga cggcgggagc gtggcagtcc tgcctgtggt
      241 cgactccacc aatcagtacc tgctggatcg aatcggcgag ctgaagagtg gggatgcttg
      301 cattgcagaa tatcagcagg cagggagagg aagcagaggg aggaaatggt tctctccttt
      361 tggagctaac ctgtacctga gtatgttttg gcgcctgaag cggggaccag cagcaatcgg
      421 cctgggcccg gtcatcggaa ttgtcatggc agaagcgctg cgaaagctgg gagcagacaa
      481 ggtgcgagtc aaatggccca atgacctgta tctgcaggat agaaagctgg caggcatcct
      541 ggtggagctg gccggaataa caggcgatgc tgcacagatc gtcattggcg ccgggattaa
      601 cgtggctatg aggcgcgtgg aggaaagcgt ggtcaatcag ggctggatca cactgcagga
      661 agcagggatt aacctggaca ggaatactct ggccgctatg ctgatccgag agctgcgggc
      721 agccctggaa ctgttcgagc aggaaggcct ggctccatat ctgtcacggt gggagaagct
      781 ggataacttc atcaatagac ccgtgaagct gatcattggg gacaaagaga ttttcgggat
      841 tagccggggg attgataaac agggagccct gctgctggaa caggacggag ttatcaaacc
      901 ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag gctagcggca agcccatccc
      961 caaccccctg ctgggcctgg acagcaccac aactttgtac aaaaaagcag gcttaatgtc
     1021 gtcgggctaa
//
