LOCUS       sequence-260359-        1165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1165)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-BRSK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..1165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             123..164
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    165..189
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggcatgatg tcgggattag ccgggggatg ataaacaggg agccctgctg ctggaacagg
       61 acggagttat caaaccctgg atgggcggag aaatcagtct gcggtctgcc gaaaaggcta
      121 gcggcaagcc catccccaac cccctgctgg gcctggacag caccacaact ttgtacaaaa
      181 aagcaggctt aatgtcgtcc ggggccaagg agggaggtgg gggctctccc gcctaccacc
      241 tcccccaccc ccacccccac ccaccccagc acgcccaata tgtgggcccc tatcggctgg
      301 agaagacgct gggcaaagga cagacagggc tggttaaact cggggtccac tgcatcacgg
      361 gtcagaaggt cgccatcaag atcgtgaacc gggagaagct gtcggagtcg gtgctgatga
      421 aggtggagcg ggagatcgcc atcctgaagc tcatcgaaca cccacatgtc ctcaagctcc
      481 acgacgtcta cgagaacaag aaatatttgt acctggttct ggagcacgtc tcggggggtg
      541 agctattcga ctacctggta aagaagggga gactgacgcc caaggaggcc cgaaagttct
      601 tccgccagat tgtgtctgcg ctggacttct gccacagcta ctccatctgc cacagagacc
      661 taaaagcccg agaacctgct tttggatgag aaaaacaaca tccgcattgc agacttcggc
      721 atggcgtccc tgcagtgggg gacagcctcc tggagaccag ctgcgggtcc tcccattatg
      781 cgtgtcagag gtgattaagg gggaaaatat gatggccgcc ggccgacagt gagctgtgag
      841 tcatctcttc gccctgctcg gggggctctg ccctttgatg acgacaactc cgacagctgc
      901 tggagaagtg gaacgaggcg cttcactgcc tcactcattc ctcaaatggc cgagcctctg
      961 gaggatgatc aatgaccgaa aagctcatct ggacaattca aacatccttg tactagcgga
     1021 aacagccaaa ctggctggag ccnctctggc ctcgtaacat gngaactcgc atccaggaac
     1081 tgacctagct cctaagagtg nnncttgggc tnnaccnnag gtgctcnatg ccnngagtan
     1141 nacagtaata tgttcgttgg tacga
//
