LOCUS       sequence-260361-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK1 (human) tagged with N-terminal V5
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-BRSK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(140..160)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             164..432
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             442..483
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    481..505
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggagngatg cggtgtgttt tgtgagtttt ttaggcacct tttgaaatgt aatcatttgg
       61 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt
      121 ggcttttttg ttagacggcc aacttttgta tacaaagtgg cgaggggctc gcatctctcc
      181 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
      241 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
      301 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
      361 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
      421 ccgttacaga tcggcaccat gggtaagcct atccctaacc ctctcctcgg tctcgattct
      481 acaactttgt acaaaaaagc aggcttaatg tcgtccgggg ccaaggaggg aggtgggggc
      541 tctcccgcct accacctccc ccacccccac ccccacccac cccagcacgc ccaatatgtg
      601 ggcccctatc ggctggagaa gacgctgggc aaaggacaga cagggctggt taaactcggg
      661 gtccactgca tcacgggtca caaggtctcc atcaagatcg tgaaccggga gaagctgtcg
      721 gactcggtgc tgatgaaggt ggagcgggac atcgtcatcc tgaagctcat cgaacactca
      781 catgtcctca agctccacga cgtctaaaaa aacaagaaat atttgtacct ggttctggag
      841 cacgtctcgg gggggtgagc tattcgcact acctggttaa gaacggagac tgactcccaa
      901 ggaggccaga agttattccg ccagattgtg tctgcgctgg aattctggca cactactcat
      961 ttgccacaga gaccttagcc ccgatacctg cttttggatg aaaaaatcac atccgccttg
     1021 cgaacttcgc atggcgtcct tgcaggtggg ggacagcttc ttgcaaccaa cggcgggtcc
     1081 ctcccataga
//
