LOCUS       sequence-260365-        1227 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1227)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..1227
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             127..168
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    169..193
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggnggcgaga ttttcgggat tagccggggg attgataaac agggagccct gctgctggaa
       61 caggacggag ttatcaaacc ctggatgggc ggagaaatca gtctgcggtc tgccgaaaag
      121 gctagcggca agcccatccc caaccccctg ctgggcctgg acagcaccac aactttgtac
      181 aaaaaagcag aagggccgtc aaggcccacc atgacatcga cggggaagga cggcggcgcg
      241 cagcacgcgc agtatgttgg gccctaccgg ctggagaaga cgctgggcaa ggggcagaca
      301 ggtctggtga agctgggggt tcactgcgtc acctgccaga aggtggccat caagatcgtc
      361 aaccgtgaga agctcagcga gtcggtgctg atgaaggtgg agcgggagat cgcgatcctg
      421 aagctcattg agcaccccca cgtcctaaag ctgcacgacg tttatgaaaa caaaaaatat
      481 ttgtacctgg tgctagaaca cgtgtcaggt ggtgagctct tcgactacct ggtgaagaag
      541 gggaggctga cgcctaagga ggctcggaag ttcttccggc agatcatctc tgcgctggac
      601 ttctgccaca gccactccat atgccacagg gatctgaaac ctgaaaacct cctgctggac
      661 gagaagaaca acatccgcat cgcagacttt ggcatggcgt ccctgcaggt tggcgacagc
      721 ctgttggaga ccagctgtgg gtccccccac tacgcctgcc ccgaggtgat ccggggggag
      781 aagtatgacg gccggaaggc ggacgtgtgg agctgcggcg tcatcctgtt cgccttgctg
      841 gtgggggctc tgcccttcga cgatgacaac ttgcgacagc tgctggagaa ggtgaagcgg
      901 ggcgtgttcc acatgccgca ctttatcccg cccgactgcc agagtctgct acggggcatg
      961 atcgaggtgg acgccgcacg ccgcctcacg ctagagcaca ttcgaaaaca catatggtat
     1021 tatagggggc aggatgagcc cgaatcagag cagcccattc ctcgcaaggt gagatcgctc
     1081 gctgcccagc tggaggacat cgaccgacgt gcttgacggc atgcatccac tgggctgctt
     1141 tcgagaccnn caggctgctg cagaacttgc tgtccgaggg aggaaaccag gaagaaagan
     1201 ttacttccat cactctggaa cacgaat
//
