LOCUS       sequence-260367-        1211 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal 
            TurboID-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1211)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-TurboID-V5-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..1211
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(136..156)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      157..166
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cnggatgcgg tgtgttttgt gagtttttta ggcacctttt gaatgtaatc atttgggtca
       61 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
      121 tttttgttag acggccaact tttgtataca aagtgggcca ccatgggaaa agacaatact
      181 gtgcctctga agctgatcgc tctcctggct aatggcgagt tccatagtgg cgaacagctg
      241 ggagaaaccc tgggcatgtc cagggccgct atcaacaagc acattcagac tctgcgcgac
      301 tggggcgtgg acgtgttcac cgtgcccgga aagggctact ctctgcccga gcctatcccg
      361 ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct
      421 gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat
      481 gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct
      541 ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca
      601 atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca
      661 gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc
      721 atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg
      781 attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg
      841 cacgaagcag gattaacctg gacaggaata ctctggccgc tacgctgatc cgagagctgc
      901 gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgcca cggtgggaga
      961 agctggatac ttcatcatag acccgtgaag ctgatcattg gggacaagag attttcggga
     1021 ttagccgggg attgataaca ggagccctgc tgctggacag acgagtatca accctgatgg
     1081 gcgagaatca gtctgcgtct gcgaaagcta gcgcagccat tccaccctgc tgctgnacgc
     1141 accaactgta caaaagcgag gcntcagcnc nngactcacg nagacgcgnn cannnncccc
     1201 antatgtttg g
//
