LOCUS       sequence-260368-        1257 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal 
            TurboID-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1257)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-TurboID-V5-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..1257
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             124..165
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    166..190
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggggaggat ttcgggatta gccgggggat gataaacagg gagccctgct gctggaacag
       61 gacggagtta tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaaggct
      121 agcggcaagc ccatccccaa ccccctgctg ggcctggaca gcaccacaac tttgtacaaa
      181 aaagcagaag ggccgtcaag gcccaccatg acatcgacgg ggaaggacgg cggcgcgcag
      241 cacgcgcagt atgttgggcc ctaccggctg gagaagacgc tgggcaaggg gcagacaggt
      301 ctggtgaagc tgggggttca ctgcgtcacc tgccagaagg tggccatcaa gatcgtcaac
      361 cgtgagaagc tcagcgagtc ggtgctgatg aaggtggagc gggagatcgc gatcctgaag
      421 ctcattgagc acccccacgt cctaaagctg cacgacgttt atgaaaacaa aaaatatttg
      481 tacctggtgc tagaacacgt gtcaggtggt gagctcttcg actacctggt gaagaagggg
      541 aggctgacgc ctaaggaggc tcggaagttc ttccggcaga tcatctctgc gctggacttc
      601 tgccacagcc actccatatg ccacagggat ctgaaacctg aaaacctcct gctggacgag
      661 aagaacaaca tccgcatcgc agactttggc atggcgtccc tgcaggttgg cgacagcctg
      721 ttggagacca gctgtgggtc cccccactac gcctgccccg aggtgatccg gggggagaag
      781 tatgacggcc ggaaggcgga cgtgtggagc tgcggcgtca tcctgttcgc cttgctggtg
      841 ggggctctgc ccttcgacga tgacaacttg cgacagctgc tggagaaggt gaagcggggc
      901 gtgttccaca tgccgcactt tatcccgccc gactgccaga gtctgctacg gggcatgatc
      961 gagggggacg ccgcacgccg cctcacgcta gagcacattc agaaacacat atgggtatat
     1021 aggggggcaa gaatgagccc gaccagagca gcccattcct cgcaaggtgc agatccgcct
     1081 cgctgcccag ccctggagga catcgacccc gacgtgctgg acagcatgca ctcactgggc
     1141 tgctcgagac gcacagctgc tgcagacngc tgtcgagaag ananccagga ggagaagntn
     1201 cttcnctcng nncgnaagaa gttaccgaag acccaggnag atggaggaac ctgtgtg
//
