LOCUS       sequence-260370-        1186 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal V5
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1186)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..1186
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(140..160)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             164..432
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             442..483
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    481..505
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gggctggctg cggtgtgttt tgtgagtttt ttaggcacct tttgaaatgt aatcatttgg
       61 gtcaatatgt aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt
      121 ggcttttttg ttagacggcc aacttttgta tacaaagtgg cgaggggctc gcatctctcc
      181 ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc cggttgagtc gcgttctgcc
      241 gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc taggtaagtt taaagctcag
      301 gtcgagaccg ggcctttgtc cggcgctccc ttggagccta cctagactca gccggctctc
      361 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
      421 ccgttacaga tcggcaccat gggtaagcct atccctaacc ctctcctcgg tctcgattct
      481 acaactttgt acaaaaaagc agaagggccg tcaaggccca ccatgacatc gacggggaag
      541 gacggcggcg cgcagcacgc gcagtatgtt gggccctacc ggctggagaa gacgctgggc
      601 aaggggcaga caggtctggt gaagctgggg gttcactgcg tcacctgcca gaaggtggcc
      661 atcaagatcg tcaaccgtga gaagctcagc gagtcggtgc tgatgaaggt ggagcgggag
      721 atcgcgatcc tgaagctcat tgagcacccc cacgtcctaa agctgcacga cgtttatgaa
      781 aacaaaaaat atttgtacct ggtgctagaa cacgtgtcag gtggtgagct cttcgactac
      841 ctggtgaaga aggggaggct gacgcctaac gaggctcgga agttcttccg gcagatcatc
      901 tctgcgctgg acttctgcca cagccactcc atatgccaca gggatctgaa acctgaaaac
      961 ctcctgctgg acgagaagaa caacatccgc atcgcagact ttggcatggc gtccctgcag
     1021 gttggcgaca gcctgttgga gaccagctgt gggatccctc ccactacgcc ctgcccgagg
     1081 tgatccgggg ggagaagtaa tgaccgtctg aagccgacgt tgtgaagctg cggccttcat
     1141 cctgtttcgc ccttgcctgg gtggggggct ctgccccttc gacnna
//
