LOCUS       sequence-260372-        8795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            N-terminal Flag for generating protein-protein networks using fusion
            tag affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8795)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 8795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-hcRed_IDG-K
FEATURES             Location/Qualifiers
     source          1..8795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2899..3582
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(3584..3608)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          3682..3911
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     3894..3913
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(3969..4543,4544..4546,4547..4555)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4136..4153)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4363..4382
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4575..5174
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4575..4594)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5071..5091
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5189..5777
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5242..5262)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5660..5671)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5852..6085
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6204..6222)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6290..6394
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6395..6463,6464..7255)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6613..6632)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7426..8014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7915..7934
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8168..8185
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8425..8446
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8461..8466,8467..8484,8485..8491)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8499..8515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8504..8526
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8523..8539
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8523..8539
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8589..8608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgtaccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag ttggcaccat ggtgagcggc ctgctgaagg agagtatgcg catcaagatg
     2941 tacatggagg gcaccgtgaa cggccactac ttcaagtgcg agggcgaggg cgacggcaac
     3001 cccttcgccg gcacccagag catgagaatc cacgtgaccg agggcgcccc cctgcccttc
     3061 gccttcgaca tcctggcccc ctgctgcgag tacggcagca ggaccttcgt gcaccacacc
     3121 gccgagatcc ccgacttctt caagcagagc ttccccgagg gcttcacctg ggagagaacc
     3181 accacctacg aggacggcgg catcctgacc gcccaccagg acaccagcct ggagggcaac
     3241 tgcctgatct acaaggtgaa ggtgcacggc accaacttcc ccgccgacgg ccccgtgatg
     3301 aagaacaaga gcggcggctg ggagcccagc accgaggtgg tgtaccccga gaacggcgtg
     3361 ctgtgcggcc ggaacgtgat ggccctgaag gtgggcgacc ggcacctgat ctgccaccac
     3421 tacaccagct accggagcaa gaaggccgtg cgcgccctga ccatgcccgg cttccacttc
     3481 accgacatcc ggctccagat gctgcggaag aagaaggacg agtacttcga gctgtacgag
     3541 gccagcgtgg cccggtacag cgacctgccc gagaaggcca acttgccaac tttcttgtac
     3601 aaagtggttg attaagatcc actagtaacg gccgccagtg tgctggaatt aattcgctgt
     3661 ctgcgagggc cagctgttgg ggtgagtact ccctctcaaa agcgggcatg acttctgcgc
     3721 taagattgtc agtttccaaa aacgaggagg atttgatatt cacctggccc gcggtgatgc
     3781 ctttgagggt ggccgcgtcc atctggtcag aaaagacaat ctttttgttg tcaagcttga
     3841 ggtgtggcag gcttgagatc tggccataca cttgagtgac aatgacatcc actttgcctt
     3901 tctctccaca ggtgtccact cccaggtcca actgcaggtc gagcatgcat ctagggcggc
     3961 caattccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     4021 ggccggtgtg cgtttgtcta tatgtgattt tccaccatat tgccgtcttt tggcaatgtg
     4081 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     4141 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     4201 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     4261 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     4321 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     4381 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     4441 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     4501 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa
     4561 ggagacgacc ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4621 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4681 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4741 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     4801 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     4861 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     4921 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     4981 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     5041 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     5101 tcaccgtcac cgccgacgtc gagtgcccga aggaccgcgc gacctggtgc atgacccgca
     5161 agcccggtgc ctgatatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag
     5221 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5281 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5341 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5401 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     5461 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5521 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5581 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5641 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5701 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5761 ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat
     5821 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5881 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5941 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6001 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6061 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     6121 taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct
     6181 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     6241 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     6301 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     6361 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     6421 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6481 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6541 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6601 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     6661 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6721 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6781 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6841 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6901 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6961 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7021 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7081 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7141 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7201 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7261 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     7321 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7381 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7441 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7501 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7561 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7621 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7681 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7741 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7801 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7861 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7921 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7981 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8041 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8101 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8161 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     8221 ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt
     8281 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8341 tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa
     8401 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     8461 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     8521 cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca
     8581 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     8641 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     8701 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     8761 aaattgtatt tgttaaatat gtactacaaa cttag
//
