LOCUS       sequence-260373-        1195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway destination clone of hcRed (as control) tagged with 
            N-terminal Flag for generating protein-protein networks using fusion
            tag affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1195)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-hcRed_IDG-K
FEATURES             Location/Qualifiers
     source          1..1195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    156..180
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             183..866
                     /codon_start=1
                     /product="far-red fluorescent variant of Heteractis crispa 
                     chromoprotein"
                     /label=HcRed1
                     /note="mammalian codon-optimized"
                     /translation="MVSGLLKESMRIKMYMEGTVNGHYFKCEGEGDGNPFAGTQSMRIH
                     VTEGAPLPFAFDILAPCCEYGSRTFVHHTAEIPDFFKQSFPEGFTWERTTTYEDGGILT
                     AHQDTSLEGNCLIYKVKVHGTNFPADGPVMKNKSGGWEPSTEVVYPENGVLCGRNVMAL
                     KVGDRHLICHHYTSYRSKKAVRALTMPGFHFTDIRLQMLRKKKDEYFELYEASVARYSD
                     LPEKAN"
     protein_bind    complement(868..892)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggatgggagc atatagcaga gctcgtttag tgaccgtcag atcgcctgga gacgccatcc
       61 acgctgtttt gacctccata gaagacaccg gcggccatac cggtcagctc gaggccacca
      121 tggactacaa ggacgacgac gacaagggcg gatcaacaag tttgtacaaa aaagttggca
      181 ccatggtgag cggcctgctg aaggagagta tgcgcatcaa gatgtacatg gagggcaccg
      241 tgaacggcca ctacttcaag tgcgagggcg agggcgacgg caaccccttc gccggcaccc
      301 agagcatgag aatccacgtg accgagggcg cccccctgcc cttcgccttc gacatcctgg
      361 ccccctgctg cgagtacggc agcaggacct tcgtgcacca caccgccgag atccccgact
      421 tcttcaagca gagcttcccc gagggcttca cctgggagag aaccaccacc tacgaggacg
      481 gcggcatcct gaccgcccac caggacacca gcctggaggg caactgcctg atctacaagg
      541 tgaaggtgca cggcaccaac ttccccgccg acggccccgt gatgaagaac aagagcggcg
      601 gctgggagcc cagcaccgag gtggtgtacc ccgagaacgg cgtgctgtgc ggccggaacg
      661 tgatggccct gaaggtgggc gaccggcacc tgatctgcca ccactacacc agctaccgga
      721 gcaagaaggc cgtgcgcgcc ctgaccatgc ccggcttcca cttcaccgac atccggctcc
      781 agatgctgcg gaagaagaag gacgagtact tcgagctgta cgaggccagc gtggcccggt
      841 acagcgacct gcccgagaag gccaacttgc caactttctt gtacaaagtg gttgattaag
      901 atccactagt aacggccgcc agtgtgctgg aattaattcg ctgtctgcga gggccagctg
      961 ttggggtgag tactccctct caaaagcggg catgacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gccgcgtgat gcctttgagg tggcgcgtca
     1081 tctggtcaga agacatcttt tgttgtcagc tgagtgtggc agctgagatc tgccatacac
     1141 tgangacatg acatccactt gctttctctc cacagggtgt tccactcccc aagtc
//
