LOCUS       sequence-260375-        8831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of GFP (as control) tagged with N-terminal
            Flag for generating protein-protein networks using fusion tag 
            affinity-based proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8831)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 8831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-CMV-Flag-GFP_IDG-K
FEATURES             Location/Qualifiers
     source          1..8831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             5..638
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    685..810
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1307..1540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1725..1769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2062..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2231..2534
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2535..2738
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2689..2709
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2830..2839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2839..2862
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2872..2896
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2899..2901,2902..2904,2905..3615)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2944..2965)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3205..3224)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3552..3573
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3620..3644)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          3718..3947
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     3930..3949
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(4005..4579,4580..4582,4583..4591)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4172..4189)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4399..4418
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4611..5210
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(4611..4630)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5107..5127
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5225..5813
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5278..5298)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5696..5707)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5888..6121
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6240..6258)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6326..6430
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6431..6499,6500..7291)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6649..6668)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7462..8050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7951..7970
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8204..8221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8461..8482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8497..8502,8503..8520,8521..8527)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8535..8551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8540..8562
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8559..8575
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8559..8575
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8625..8644
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
       61 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
      121 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
      181 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
      241 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
      301 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
      361 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
      421 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
      481 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      541 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      601 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      661 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      721 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      781 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      841 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      901 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      961 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1021 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1081 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1141 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1201 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1261 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1321 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1381 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1441 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1501 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1561 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1621 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1681 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1741 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1801 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1861 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1921 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1981 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca
     2041 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2101 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2161 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggactag
     2221 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2281 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2341 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2401 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2461 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2521 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2581 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2641 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2701 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccggcgg ccgtaccggt
     2821 cagctcgagg ccaccatgga ctacaaggac gacgacgaca agggcggatc aacaagtttg
     2881 tacaaaaaag caggcttaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2941 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc tggcgagggc
     3001 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     3061 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     3121 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     3181 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     3241 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     3301 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     3361 gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgaggac
     3421 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     3481 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     3541 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3601 gacgagctgt acaagtccaa cccagctttc ttgtacaaag tggttgatta agatccacta
     3661 gtaacggccg ccagtgtgct ggaattaatt cgctgtctgc gagggccagc tgttggggtg
     3721 agtactccct ctcaaaagcg ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg
     3781 aggaggattt gatattcacc tggcccgcgg tgatgccttt gagggtggcc gcgtccatct
     3841 ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc
     3901 catacacttg agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca
     3961 ggtccaactg caggtcgagc atgcatctag ggcggccaat tccgcccctc tccctccccc
     4021 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     4081 tgattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     4141 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     4201 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     4261 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     4321 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     4381 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     4441 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     4501 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     4561 tgaaaaacac gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt
     4621 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     4681 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     4741 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4801 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     4861 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     4921 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     4981 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     5041 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     5101 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagt
     5161 gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga tatcgataga
     5221 tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5281 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5341 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5401 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5461 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5521 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5581 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     5641 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5701 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5761 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat
     5821 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5881 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     5941 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6001 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6061 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6121 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6181 gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg
     6241 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     6301 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     6361 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     6421 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6481 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6541 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6601 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6661 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6721 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6781 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6841 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6901 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6961 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     7021 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     7081 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     7141 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     7201 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     7261 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     7321 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     7381 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     7441 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7501 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7561 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7621 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7681 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7741 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7801 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7861 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7921 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7981 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     8041 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     8101 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     8161 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     8221 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     8281 atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     8341 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     8401 aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     8461 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     8521 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
     8581 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8641 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     8701 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     8761 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     8821 tacaaactta g
//
